HtrA1 serine protease expression levels on age-related macular degeneration (AMD) patients in Yogyakarta
| Autor: | Tri Wahyu Widayanti, Muhammad Bayu Sasongko, Firman Setya Wardhana, Anindita Dianratri, Mohammad Eko Prayogo, Chio Oka, Anditta Syifarahmah, Ayudha Bahana Ilham Perdamaian, Supanji Supanji, Angela Nurini Agni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Environmental Engineering genetic structures lcsh:QR1-502 Gastroenterology Industrial and Manufacturing Engineering lcsh:Microbiology lcsh:Physiology 03 medical and health sciences 0302 clinical medicine Internal medicine Age related Genotype lcsh:Zoology medicine lcsh:QL1-991 Serine protease biology lcsh:QP1-981 business.industry Incidence (epidemiology) Elisa assay Macular degeneration medicine.disease eye diseases 030104 developmental biology 030220 oncology & carcinogenesis HTRA1 biology.protein sense organs business Statistical correlation |
| Zdroj: | BIO Web of Conferences, Vol 28, p 02004 (2020) |
| ISSN: | 2117-4458 |
| Popis: | This research aims to investigate the HtrA1 serine protease circulating level of Age-related Macular Degeneration (AMD) patients in Yogyakarta, Indonesia. This study was conducted from January to August 2019 which included 38 AMD patients and 16 Non-AMD patients/controls (two groups). Baseline data and blood sample were collected. ELISA assay was used to measure the HtrA1 serine protease circulating level on both groups. SNP genotyping of rs10490924 was using restriction enzyme digestion. This study used The IBM SPSS® version 24 (Chicago, The USA) to determine the relationship between HtrA1 expression level and AMD incidence. AMD patients had higher HtrA1 serine protease level (35.31) than controls (30.08). However, there is no association found between HtrA1 serine protease level and AMD incidence (p-value>0.05, CI 95 %). However, HtrA1 serine protease did not associate positively to AMD incidence in Yogyakarta samples. Further analysis by grouping AMD patient based on the rs10490924 genotype show no statistical correlation between HTRA1 to the incidence of AMD. This result might be due to the lack of samples in the study groups. Future studies with larger number of samples are advised to better see the association between Htra1 serine protease level and AMD incidence. |
| Databáze: | OpenAIRE |
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