Interleukin-7 matures suppressive CD127(+) forkhead box P3 (FoxP3)(+) T cells into CD127(-) CD25(high) FoxP3(+) regulatory T cells
| Autor: | Massimo Trucco, Nick Giannoukakis, Robert Lakomy, C. Engman, Jo Harnaha, Brett E. Phillips, Antonella D'Anneo, V. Di Caro, A. Styche |
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| Přispěvatelé: | Di Caro, V, D'Anneo, A, Phillips, B, Engman, C, Harnaha, J, Lakomy, R, Styche, A, Trucco, M, Giannoukakis, N |
| Rok vydání: | 2011 |
| Předmět: |
Translational Studies
T cell Immunology Active Transport Cell Nucleus chemical and pharmacologic phenomena Biology T-Lymphocytes Regulatory Interleukin-7 Receptor alpha Subunit Interleukin 21 Mice Antigen Antigens CD T-Lymphocyte Subsets medicine Immunology and Allergy Cytotoxic T cell Animals CTLA-4 Antigen IL-2 receptor Interleukin-7 receptor Cells Cultured Cell Nucleus Mice Inbred BALB C Interleukin-7 autoimmunity Interleukin-2 Receptor alpha Subunit FOXP3 virus diseases hemic and immune systems Cell Differentiation Forkhead Transcription Factors T lymphocyte Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation Leukocyte Common Antigens FoxP3 Treg |
| Zdroj: | Clinical and experimental immunology. 165(1) |
| ISSN: | 1365-2249 |
| Popis: | We have identified a novel interleukin (IL)-7-responsive T cell population [forkhead box P3 (FoxP3(+) ) CD4(+) CD25(+) CD127(+) ] that is comparably functionally suppressive to conventional FoxP3(+) CD4(+) CD25(+) regulatory T cells (T(regs) ). Although IL-2 is the most critical cytokine for thymic development of FoxP3(+) T(regs) , in the periphery other cytokines can be compensatory. CD25(+) CD127(+) T cells treated with IL-7 phenotypically 'matured' into the known 'classical' FoxP3(+) CD4(+) CD25(high) CD127(-) FoxP3(+) T(regs) . In freshly isolated splenocytes, the highest level of FoxP3 expression was found in CD127(+) CD25(+) T cells when compared with CD127(-) CD25(+) or CD127(+) CD25(-) cells. IL-7 treatment of CD4(+) CD25(+) T cells induced an increase in the accumulation of FoxP3 in the nucleus in vitro. IL-7-mediated CD25 cell surface up-regulation was accompanied by a concurrent down-regulation of CD127 in vitro. IL-7 treatment of the CD127(+) CD25(+) FoxP3(+) cells also resulted in up-regulation of cytotoxic T lymphocyte antigen 4 without any changes in CD45RA at the cell surface. Collectively, these data support emerging evidence that FoxP3(+) T cells expressing CD127 are comparably functionally suppressive to CD25(+) CD127(-) FoxP3(+) T cells. This IL-7-sensitive regulation of FoxP3(+) T(reg) phenotype could underlie one peripheral non-IL-2-dependent compensatory mechanism of T(reg) survival and functional activity, particularly for adaptive T(regs) in the control of autoimmunity or suppression of activated effector T cells. |
| Databáze: | OpenAIRE |
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