A simple technique for determination of P50 values of blood and erythrocytes

Autor: David J. Newman, Edward G. Brann, William E. Maya, Deborah S. Seltz, Diane P. Naselsky
Rok vydání: 1973
Předmět:
Zdroj: Biochemical Medicine. 8:72-77
ISSN: 0006-2944
DOI: 10.1016/0006-2944(73)90010-0
Popis: A simple, rapid, and repetitive technique for determining the P 50 value of blood or erythrocyte suspension is described. The system allows comparative values of various blood samples or treatments to be obtained under the same gassing and equilibration conditions. For a series of normal human erythrocytes in physiologic buffer ( n = 37), P 50 = 27.45 ± 1.34 mm and on sextuple analyses of one sample, P 50 = 28.40 ± 0.50 mm. A computer program is given that converts raw data to P 50 values at pH 7.40.
Databáze: OpenAIRE