Development of an ionic-liquid-based dispersive liquid-liquid microextraction method for the determination of antichagasic drugs in human breast milk: Optimization by central composite design

Autor:	Mario Reta, Romina Noel Echevarria, Juan Manuel Padró, Alicia Noemi Califano, Rocio Pellegrino Vidal
Rok vydání:	2015
Předmět:	Central composite design Liquid Phase Microextraction Ultraviolet Rays antichagasic drugs Filtration and Separation Breast milk Analytical Chemistry ionic liquids chemistry.chemical_compound dispersive liquid–liquid microextraction medicine Humans liquid chromatography Chagas Disease central composite design Nifurtimox Chromatography High Pressure Liquid Detection limit Chromatography Milk Human Molecular Structure Chemistry Imidazoles Química Trypanocidal Agents Solvent Nitroimidazoles Benznidazole Ionic strength Ionic liquid medicine.drug
Zdroj:	CIC Digital (CICBA) Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA
ISSN:	1615-9306
DOI:	10.1002/jssc.201401367
Popis:	Chagas disease constitutes a major public health problem in Latin America. Human breast milk is a biological sample of great importance for the analysis of therapeutic drugs, as unwanted exposure through breast milk could result in pharmacological effects in the nursing infant. Thus, the goal of breast milk drug analysis is to inquire to which extent a neonate may be exposed to a drug during lactation. In this work, we developed an analytical technique to quantify benznidazole and nifurtimox (the two antichagasic drugs currently available for the medical treatment) in human breast milk, with a simple sample pre-treatment followed by an ionic-liquid-based dispersive liquid–liquid microextraction combined with high-performance liquid chromatography and UV detection. For this technique, the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate has been used as the “extraction solvent”. A central composite design was used to find the optimum values for the significant variables affecting the extraction process: volume of ionic liquid, volume of dispersant solvent, ionic strength, and pH. At the optimum working conditions, the average recoveries were 77.5 and 89.7%, the limits of detection were 0.06 and 0.09 μg mL-1 and the inter-day reproducibilities were 6.25 and 5.77% for benznidazole and nifurtimox, respectively. The proposed methodology can be considered sensitive, simple, robust, accurate, and green. Universidad Nacional de La Plata (UNLP) - Facultad de Ciencias Exactas
Databáze:	OpenAIRE
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