Decreased Levels of Folate Receptor-β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre-Eclampsia

Autor:	Errol R. Norwitz, Seth Guller, Catalin S. Buhimschi, Se-Te J. Huang, Zhonghua Tang, Irina A. Buhimschi, Serkalem Tadesse, Tracy Niven-Fairchild
Rok vydání:	2013
Předmět:	Adult medicine.medical_specialty Placenta Immunology Antigens Differentiation Myelomonocytic Down-Regulation Gene Expression Receptors Cell Surface Biology Article Young Adult Pre-Eclampsia Antigens CD Pregnancy Internal medicine medicine Humans Immunology and Allergy Folate Receptor 2 RNA Messenger Fetus Eclampsia CD68 Gene Expression Profiling Macrophages Obstetrics and Gynecology medicine.disease Endocrinology medicine.anatomical_structure Real-time polymerase chain reaction Reproductive Medicine Gestation Female CD163
Zdroj:	American Journal of Reproductive Immunology. 70:104-115
ISSN:	1046-7408
DOI:	10.1111/aji.12112
Popis:	Problem Pre-eclampsia (PE), a pregnancy complication of unknown etiology, is a major cause of maternal and fetal mortality and morbidity. Previous studies have described placental genes that are up-regulated in expression in PE, but few studies have addressed placental gene suppression in this syndrome. Method of study Gene profiling and quantitative reverse transcription PCR (qRTPCR) analyses were used to identify genes down-regulated in placentas from women with severe preterm PE compared with gestational age-matched normotensive controls with spontaneous preterm birth (sPTB). Western blotting and immunohistochemistry were used to evaluate levels and patterns of cell type–specific protein expression in PE and sPTB group placentas. Results Levels of macrophage marker [folate receptor (FR)-β, CD163, and CD68] mRNA and FR-β protein were significantly down-regulated in PE group placentas compared with the sPTB group. Numbers of Hofbauer cells (HBCs, fetal macrophages) and FR-β protein in these cells were reduced in PE group placentas. Conclusion Severe PE is associated with decreased placental expression of FR-β and a reduction in the number of HBCs. Reduced placental macrophage function is likely to play a key role in the pathophysiology of PE.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc46534409baae7b1678f986f401edda https://doi.org/10.1111/aji.12112 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.