Imaging the Hydrated Microbe-Metal Interface Using Nanoscale Spectrum Imaging
| Autor: | Eric Prestat, Edward A. Lewis, Jonathan R. Lloyd, Sarah J. Haigh, Helen F. Downie, Richard F. Collins |
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| Rok vydání: | 2016 |
| Předmět: |
Nanostructure
Materials science Population Analytical chemistry Nanoparticle Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention law Manchester Institute of Biotechnology Scanning transmission electron microscopy General Materials Science education Nanoscopic scale Bimetallic strip Geobacter sulfurreducens education.field_of_study biology General Chemistry ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Electron microscope 0210 nano-technology |
| Zdroj: | Lewis, E, Haigh, S, Downie, H, Collins, R, Prestat, E & Lloyd, J 2016, ' Imaging the hydrated microbe-metal interface using nanoscale spectrum imaging ', Particle and Particle Systems Characterization . https://doi.org/10.1002/ppsc.201600073 |
| ISSN: | 0934-0866 |
| DOI: | 10.1002/ppsc.201600073 |
| Popis: | PdAu nanocrystals were synthesised by Geobacter sulfurreducens, a dissimilatory metalreducing bacterium, and the resulting bimetallic nanocrystal decorated microbes were imaged using a range of advanced electron microscopy techniques. Specifically we report the first example of elemental mapping of fully hydrated biological nanostructures using scanning transmission electron microscope (STEM) energy dispersive X-ray (EDX) spectrum imaging within an environmental liquid-cell. We combine these results with cryo-TEM and ex situ STEM imaging and EDX analysis with the aim of better understanding microbial synthesis of bimetallic nanoparticles. We demonstrate that although Au and Pd are colocalised across the cells, the population of nanoparticles produced is bimodal, containing ultra-small alloyed nanocrystals with diameters 200nm in diameter) which show higher Pd contents and exhibited a Pd enriched rich shell only a few nanometres thick. The application of high-resolution imaging techniques described here offers the potential to visualise the microbe-metal interface during the bioproduction of a range of functional materials by microbial “green” synthesis routes, and also key interfaces underpinning globally relevant environmental processes (e.g. metal cycling). |
| Databáze: | OpenAIRE |
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