Functional expression of human leukocyte elastase (HLE)/medullasin in eukaryotic cells
| Autor: | Yosuke Aoki, Hirohiko Shimizu, Masanobu Naruto, Kiyoshi Okano |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Genetic Vectors
Molecular Sequence Data Restriction Mapping Biophysics Gene Expression Molecular cloning Biology Transfection Biochemistry Cell Line Plasmid Complementary DNA Leukocytes Animals Humans Amino Acid Sequence Cloning Molecular Molecular Biology Base Sequence Pancreatic Elastase cDNA library Serine Endopeptidases Elastase DNA Cell Biology Fusion protein Molecular biology Cell culture Interleukin-2 sense organs Oligonucleotide Probes Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 167:1326-1332 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(90)90668-d |
| Popis: | We have cloned a full length cDNA for human leukocyte elastase (HLE, EC 3.4.21.37)/medullasin from the cDNA library of human leukemic cell line, ML3. Recombinant plasmid for the expression of HLE cDNA in eukaryotic cells was constructed in which HLE cDNA was fused in a frame to a leader sequence of human interleukin-2 (IL-2). COS-1 cells, transfected with the plasmid, secreted fusion protein consists of N-terminal 8 amino acid (aa) residues of human IL-2 and 238 aa residues of HLE. As the fusion protein was designed to be connected through lysine residue, elastase activity was generated after digestion of the fusion protein with lysyl-endopeptidase. |
| Databáze: | OpenAIRE |
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