The extracellular domain of CD83 inhibits dendritic cell-mediated T cell stimulation and binds to a ligand on dendritic cells
| Autor: | Diana Dudziak, Elisabeth Kremmer, Christine Kuhnt, Matthias Lechmann, Carl G. Figdor, Gerold Schuler, Daniëlle J. E. B. Krooshoop, Alexander Steinkasserer |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
T-Lymphocytes
T cell Immunology Antigen presentation Immunoglobulins chemical and pharmacologic phenomena recombinant expression Cell Communication Immunoglobulin domain Biology CD83 dendritic cells MLR T cell inhibition recombinant expression Ligands MLR Antigen CD83 Antigens CD Escherichia coli Extracellular medicine Humans Immunology and Allergy Immunoglobulin Fragments Cells Cultured Antigen Presentation Membrane Glycoproteins Follicular dendritic cells Cell Differentiation hemic and immune systems Dendritic Cells Dendritic cell Molecular biology Cell biology medicine.anatomical_structure T cell inhibition Original Article Tumorimmunology CD80 Haematology Protein Binding |
| Zdroj: | Journal of Experimental Medicine, 194, 12, pp. 1813-1821 Journal of Experimental Medicine, 194, 12, pp. 1813-21 Journal of Experimental Medicine, 194, 1813-21 The Journal of Experimental Medicine J. Exp. Med. 194, 1813-1821 (2001) Journal of Experimental Medicine, 194, 1813-1821. Rockefeller univ press |
| ISSN: | 0022-1007 |
| Popis: | Item does not contain fulltext CD83 is an immunoglobulin (Ig) superfamily member that is upregulated during the maturation of dendritic cells (DCs). It has been widely used as a marker for mature DCs, but its function is still unknown. To approach its potential functional role, we have expressed the extracellular Ig domain of human CD83 (hCD83ext) as a soluble protein. Using this tool we could show that immature as well as mature DCs bind to CD83. Since CD83 binds a ligand also expressed on immature DCs, which do not express CD83, indicates that binding is not a homophilic interaction. In addition we demonstrate that hCD83ext interferes with DC maturation downmodulating the expression of CD80 and CD83, while no phenotypical effects were observed on T cells. Finally, we show that hCD83ext inhibits DC-dependent allogeneic and peptide-specific T cell proliferation in a concentration dependent manner in vitro. This is the first report regarding functional aspects of CD83 and the binding of CD83 to DCs. |
| Databáze: | OpenAIRE |
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