Lack of correlated expression between the glutathione S-transferase P-form and the oncogene products c-Jun and c-Fos in rat tissues and preneoplastic hepatic foci
| Autor: | Ichiro Hatayama, Shinsaku Suzuki, Shigeki Tsuchida, Kimihiko Satoh, Hajime Nakano, Kiyomi Sato |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Oncogene Proteins Proto-Oncogene Proteins c-jun Recombinant Fusion Proteins Biology c-Fos Rats Sprague-Dawley Liver Neoplasms Experimental Antibody Specificity Gene expression medicine Animals Glutathione Transferase Oncogene c-jun General Medicine Immunohistochemistry Epithelium Rats Isoenzymes Glutathione S-transferase medicine.anatomical_structure biology.protein Precancerous Conditions Proto-Oncogene Proteins c-fos |
| Zdroj: | Carcinogenesis. 16:567-571 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/16.3.567 |
| Popis: | Since the expression of glutathione S-transferase P-form (GST-P) has been suggested from in vitro studies to be partly regulated by the oncogene products, c-Jun and c-Fos, their distributions were compared in normal rat tissues and preneoplastic hepatic lesions induced by the Solt-Farber protocol. Immunohistochemically demonstrated GST-P protein was positively correlated with expression of both c-Jun and c-Fos in the epidermis of the skin and the smooth muscle of adult lung and with either c-Jun or c-Fos respectively in the bile ducts and bronchial epithelium. However, GST-P expression was also observed in proximal and distal straight segments of the kidney and other tissues negative for c-Jun and c-Fos and both c-Jun and c-Fos were present in the renal proximal and distal convoluted tubules, where GST-P was lacking. Thus, the localization of GST-P was in some cases clearly separable from those of c-Jun or c-Fos. GST-P was found to be focally expressed from an early stage of hepatocarcinogen-esis, when c-Jun was not detectable. At later stages, this oncogene product was stained in 35.7% of GST-P-positive foci, with a clear relation to the degree of GST-P staining. Since GST-P is not always accompanied by appreciable c-Jun or c-Fos, these oncogene products are apparently not prerequisites for its expression. However, c-Jun may be partly responsible for maintaining high levels of GST-P in hepatic foci at later stages of hepatocarcinogenesis |
| Databáze: | OpenAIRE |
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