Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3
| Autor: | Robin J. Marjoram, Jing Xu, Alastair J. Barr, Ralph Snyderman |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
MAP Kinase Kinase 3
Biophysics Phospholipase C beta Saccharomyces cerevisiae Mitogen-activated protein kinase kinase Biochemistry p38 Mitogen-Activated Protein Kinases MAP2K7 Leukocytes Humans ASK1 Cloning Molecular Molecular Biology Protein kinase C Gene Library Mitogen-Activated Protein Kinase Kinases biology MAP kinase kinase kinase Cyclin-dependent kinase 2 Cell Biology Protein-Tyrosine Kinases Cell biology Isoenzymes Type C Phospholipases biology.protein Cyclin-dependent kinase 9 Casein kinase 2 Mitogen-Activated Protein Kinases |
| Zdroj: | Biochemical and biophysical research communications. 293(1) |
| ISSN: | 0006-291X |
| Popis: | Phospholipase C (PLC)-beta enzymes (isoenzymes beta 1-beta 4) are activated by G protein subunits, leading to the generation of intracellular messengers which mobilize calcium and activate protein kinase C. It has recently been recognized that these enzymes interact with and are regulated by proteins other than G proteins. Using the yeast two-hybrid technique to screen a leukocyte library we identified mitogen-activated protein kinase kinase 3 (MKK3) as a partner of PLC-beta 2. The interaction was confirmed by co-immunoprecipitation assays which indicated that MKK3 interacts with PLC-beta 2, but not with other PLC-betas. PLC-beta 2 interacted weakly with MKK6, which is related to MKK3, but not with the other MKK3 tested. The region of PLC-beta 2 involved in the interaction with MKK3 was mapped to the C-terminus of PLC-beta 2. p38MAPK also co-immunoprecipitated with PLC-beta 2. The data suggest that PLC-beta 2 serves an unappreciated role assembling components of the p38MAPK signaling module. |
| Databáze: | OpenAIRE |
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