Effects of colchicine-induced microtubule depolymerization on TRPV4 in rats with chronic compression of the dorsal root ganglion
| Autor: | Zhen-Zhen Fan, Hong You Ge, Yang Zhang, Wei Yao, Hong Jiang, Hui Wei, Lars Arendt-Nielsen, Shouwei Yue |
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| Rok vydání: | 2013 |
| Předmět: |
TRPV4
Patch-Clamp Techniques Cell Survival TRPV Cation Channels Pharmacology Microtubules Cell Line Transient receptor potential channel chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal medicine Animals Humans Colchicine Patch clamp Radiculopathy Reversal potential Chemistry General Neuroscience Tubulin Modulators Rats Posterior Horn Cells Allodynia medicine.anatomical_structure Hyperalgesia Cell culture Anesthesia Chronic Disease Neuralgia medicine.symptom |
| Zdroj: | Wei, H, Zhang, Y, Fan, Z-Z, Ge, H-Y, Arendt-Nielsen, L, Jiang, H, Yao, W & Yue, S-W 2013, ' Effects of colchicine-induced microtubule depolymerization on TRPV4 in rats with chronic compression of the dorsal root ganglion ', Neuroscience Letters, vol. 534, no. 1, pp. 344-350 . https://doi.org/10.1016/j.neulet.2012.12.003 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2012.12.003 |
| Popis: | The aim of this study was to investigate the effect of colchicine-induced microtubule depolymerization on allodynia in rats with chronic compression of the dorsal root ganglion (DRG) (CCD) and the effect of colchicine on transient receptor potential vanilloid 4 (TRPV4). Intrathecal administration of the anti-microtubule agent, colchicine, resulted in a dose-dependent and partial reduction in CCD-induced mechanical and thermal allodynia. The reduction of allodynia was associated with significant and dose-dependent decreases in the levels of both TRPV4 mRNA and protein expression in CCD rats. In addition, colchicine resulted in reduction and advance of TRPV4 currents in both DRG neurons and HEK293-TRPV4 cells. The current-voltage (IV) relation in HEK293-TRPV4 cells that were exposed to colchicine displayed a typical outward rectification characteristic of TRPV4 with the reversal potential shifted toward a more positive voltage. In conclusion, intrathecal administration of colchicine attenuated allodynia and TRPV4 contributed to the colchicine-induced attenuation of allodynia in CCD rats. |
| Databáze: | OpenAIRE |
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