Sterile-filtered saliva is a strong inducer of IL-6 and IL-8 in oral fibroblasts
| Autor: | Reinhard Gruber, Barbara Cvikl, Anton Sculean, Adrian Lussi, Andreas Moritz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chemokine
Saliva Connective tissue Inflammation 610 Medicine & health stomatognathic system medicine Humans Interleukin 8 General Dentistry Mouth Innate immune system biology Interleukin-6 Interleukin-9 Fibroblasts Disinfection stomatognathic diseases medicine.anatomical_structure Immunology biology.protein Signal transduction medicine.symptom Inflammation Mediators Wound healing |
| Zdroj: | Cvikl, Barbara; Lussi, Adrian; Moritz, Andreas; Sculean, Anton; Gruber, Reinhard (2015). Sterile-filtered saliva is a strong inducer of IL-6 and IL-8 in oral fibroblasts. Clinical oral investigations, 19(2), pp. 385-399. Springer 10.1007/s00784-014-1232-3 |
| DOI: | 10.1007/s00784-014-1232-3 |
| Popis: | OBJECTIVES Saliva has been implicated to support oral wound healing, a process that requires a transient inflammatory reaction. However, definitive proof that saliva can provoke an inflammatory response remained elusive. MATERIALS AND METHODS We investigated the ability of freshly harvested and sterile-filtered saliva to cause an inflammatory response of oral fibroblasts and epithelial cells. The expression of cytokines and chemokines was assessed by microarray, RT-PCR, immunoassays, and Luminex technology. The involvement of signaling pathways was determined by Western blot analysis and pharmacologic inhibitors. RESULTS We report that sterile-filtered whole saliva was a potent inducer of IL-6 and IL-8 in fibroblasts from the gingiva, the palate, and the periodontal ligament, but not of oral epithelial cells. This strong inflammatory response requires nuclear factor-kappa B and mitogen-activated protein kinase signaling. The pro-inflammatory capacity is heat stable and has a molecular weight of |
| Databáze: | OpenAIRE |
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