Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis
| Autor: | Mari-Cruz Castillo, Álvaro Costa-Broseta, José León |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Ciencia, Innovación y Universidades (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cysteine S-nitrosation Ubiquitylation Metabolic Clearance Rate Nitrogen assimilation Arabidopsis 01 natural sciences Article Catalysis Nitric oxide lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Biosynthesis Nitrate Reductases Ammonium Compounds Homeostasis Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Nitrites Spectroscopy Nitrite reductase Plant growth chemistry.chemical_classification Nitrates biology Organic Chemistry Wild type General Medicine biology.organism_classification Computer Science Applications Amino acid 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Biochemistry Tyrosine nitration Nitrate assimilation Protein Processing Post-Translational 010606 plant biology & botany Cysteine |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Digital.CSIC. Repositorio Institucional del CSIC International Journal of Molecular Sciences Volume 22 Issue 2 International Journal of Molecular Sciences, Vol 22, Iss 549, p 549 (2021) |
| Popis: | Nitric oxide (NO) is a regulator of growth, development, and stress responses in living organisms. Plant nitrate reductases (NR) catalyze the reduction of nitrate to nitrite or, alternatively, to NO. In plants, NO action and its targets remain incompletely understood, and the way NO regulates its own homeostasis remains to be elucidated. A significant transcriptome overlapping between NO-deficient mutant and NO-treated wild type plants suggests that NO could negatively regulate its biosynthesis. A significant increase in NO content was detected in transgenic plants overexpressing NR1 and NR2 proteins. In turn, NR protein and activity as well as NO content, decreased in wild-type plants exposed to a pulse of NO gas. Tag-aided immunopurification procedures followed by tandem mass spectrometry allowed identifying NO-triggered post-translational modifications (PTMs) and ubiquitylation sites in NRs. Nitration of tyrosine residues and S-nitrosation of cysteine residues affected key amino acids involved in binding the essential FAD and molybdenum cofactors. NO-related PTMs were accompanied by ubiquitylation of lysine residues flanking the nitration and S-nitrosation sites. NO-induced PTMs of NRs potentially inhibit their activities and promote their proteasome-mediated degradation. This auto-regulatory feedback loop may control nitrate assimilation to ammonium and nitrite-derived production of NO under complex environmental conditions. This research was funded by BIO2014-56067-P and BIO2017-82945-P grants from the Spanish Ministry of Economy, Industry, and Competitiveness, and FEDER funds. |
| Databáze: | OpenAIRE |
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