Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment
| Autor: | Hye Seon Song, Hong Seok Son, Seung Woo Ahn, Se Hee Lee, Juseok Kim, Yoon E. Choi, Joon Yong Kim, Yeon Bee Kim, Jinjong Myoung, Yu Jin Kim, Seong Woon Roh, Tae Woong Whon, Seung Ran Yoo |
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| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Fresh Water Biology 010603 evolutionary biology 01 natural sciences Microbiology Genome 03 medical and health sciences Genus RNA Ribosomal 16S Botany Molecular Biology Phospholipids Phylogeny Base Composition Clostridiales Phylogenetic tree Strain (chemistry) Circular bacterial chromosome Fatty Acids Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques 030104 developmental biology RRNA Operon Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 114:507-513 |
| ISSN: | 1572-9699 0003-6072 |
| DOI: | 10.1007/s10482-021-01536-4 |
| Popis: | Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (= KACC 21652T = DSM 110663T). |
| Databáze: | OpenAIRE |
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