An Update on the Laboratory Diagnosis of Rickettsia spp. Infection
| Autor: | Alexandra G. A. Stewart, Adam G Stewart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Disease Computational biology Review Serology law.invention Diagnostic modalities law molecular diagnosis Immunology and Allergy Rickettsia Molecular Biology Polymerase chain reaction metagenomics spotted fever group General Immunology and Microbiology biology Transmission (medicine) biology.organism_classification Infectious Diseases Metagenomics Medicine Identification (biology) |
| Zdroj: | Pathogens Pathogens, Vol 10, Iss 1319, p 1319 (2021) |
| ISSN: | 2076-0817 |
| Popis: | Rickettsia species causing human illness are present globally and can cause significant disease. Diagnosis and identification of this intracellular bacteria are challenging with many available diagnostic modalities suffering from several shortcomings. Detection of antibodies directed against Rickettsia spp. via serological methods remains widely used with a broad range of sensitivity and specificity values reported depending on the assay. Molecular methods, including polymerase chain reaction (PCR) testing, enables species-specific identification with a fast turnaround time; however, due to resource requirements, use in some endemic settings is limited. Reports on the use of next-generation sequencing (NGS) and metagenomics to diagnose Rickettsia spp. infection have been increasing. Despite offering several potential advantages in the diagnosis and surveillance of disease, genomic approaches are currently only limited to reference and research laboratories. Continued development of Rickettsia spp. diagnostics is required to improve disease detection and epidemiological surveillance, and to better understand transmission dynamics. |
| Databáze: | OpenAIRE |
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