Rotavirus RNAs sponge host cell RNA binding proteins and interfere with their subcellular localization
| Autor: | Gustavo Martínez-Delgado, Susana López, Andrea Peralta, Carlos F. Arias, Alfonso Oceguera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rotavirus P-BODIES RNA-binding protein RNA GRANULES Biology medicine.disease_cause Antibodies Viral Cell Line purl.org/becyt/ford/1 [https] Ciencias Biológicas 03 medical and health sciences Stress granule GW-BODIES Virology Polysome P-bodies medicine Animals RNA Messenger purl.org/becyt/ford/1.6 [https] RNA RNA-Binding Proteins Subcellular localization Macaca mulatta Cell biology VIRUS HOST CELL INTERACTIONS RNA BINDING PROTEINS Protein Transport RNA SPONGES 030104 developmental biology Gene Expression Regulation Cytoplasm RNA Viral ROTAVIRUS Virología CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Cellular mRNAs cycle between translating and non-translating pools, polysomes compose the translating pool, while RNA granules contain translationally-silenced mRNAs, where the RNAs are either stored in stress granules, or accumulate in processing bodies (PBs) or GW-bodies, which have an important role in RNA degradation. Viruses have developed measures to prevent the deleterious effects of these structures during their replication. Rotavirus, the most common agent of viral gastroenteritis, is capable of establishing a successful infection by counteracting several of the antiviral responses of its host. Here, we describe that in rotavirus-infected cells the distribution of several RNA binding proteins is changed causing the disaggregation of PBs, the relocalization of GW-body proteins, and the cytoplasmic accumulation of HuR, a predominantly nuclear protein. We show that this redistribution of proteins is more likely caused by the accumulation of viral RNA in the cytoplasm of infected-cells, where it might be acting as an RBP sponge. Fil: Oceguera, Alfonso. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México Fil: Gustavo Martinez Delgado. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México. Instituto Cumbres Lomas; México Fil: Arias, Carlos F.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México Fil: López, Susana. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México |
| Databáze: | OpenAIRE |
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