Potential value of circulatory microRNA10b gene expression and its target E‐cadherin as a prognostic and metastatic prediction marker for breast cancer

Autor: Salwa Hamdi Gomaa, Fatma I. Dwedar, Ayman Farouk Mohammed, Reham Said Shams-Eldin, Salwa Nayer Mohamed
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
Oncology
Adult
medicine.medical_specialty
Clinical Biochemistry
Breast Neoplasms
Sensitivity and Specificity
Metastasis
Breast cancer
breast cancer
Downregulation and upregulation
Antigens
CD
Internal medicine
soluble E‐cadherin
Gene expression
microRNA
TaqMan
Biomarkers
Tumor
Immunology and Allergy
Medicine
circulating miR‐10b
Humans
Circulating MicroRNA
Receptors
Immunologic
Research Articles
Aged
Membrane Glycoproteins
Cadherin
business.industry
Biochemistry (medical)
Public Health
Environmental and Occupational Health
TaqMan miRNA assay
Hematology
Middle Aged
medicine.disease
Cadherins
Prognosis
Gene Expression Regulation
Neoplastic
Medical Laboratory Technology
non‐invasive biomarker
ROC Curve
Case-Control Studies
Circulatory system
Female
business
Research Article
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
Popis: Background Breast cancer (BC) is the leading cause of cancer death in women worldwide. Most BC studies on candidate microRNAs were tissue specimen based. Recently, there has been a focus on the study of cell‐free circulating miRNAs as promising biomarkers in (BC) diagnosis and prognosis. Therefore, we aimed to investigate the circulating levels of miR‐10b and its target soluble E‐ cadherin as potentially easily accessible biomarkers for breast cancer. Methods Sixty‐one breast cancer patients and forty‐eight age‐ and sex‐matched healthy volunteers serving as a control group were enrolled in the present study. Serum samples were used to assess miRNA10b expression by TaqMan miRNA assay technique. In addition, soluble E‐cadherin expression level in serum was determined using ELISA technique. Result Circulating miR‐10b expression level and serum sE‐cadherin was significantly upregulated in patients with BC compared to controls. Moreover, serum miR‐10b displayed progressive up‐regulation in advanced stages with higher level in metastatic compared to non‐metastatic BC. Additionally, the combined use of both serum miR‐10b and sE‐cadherin revealed the highest sensitivity and specificity for detection of BC metastasis (92.9% and 97.9% respectively) with an area under curve (AUC) of 0.98, 95% CI (0.958–1.00). Conclusion Our data suggest that circulating miR‐10b could be utilized as a potential non‐invasive serum biomarker for diagnosis and prognosis of breast cancer with better performance to predict BC metastasis achieved on measuring it simultaneously with serum sE‐cadherin. Further studies with a large cohort of patients are warranted to validate the serum biomarker for breast cancer management.
Our results emphasize the oncogenic role of miR‐10b and indicate that its high serum expression may be correlated with poor outcome in breast cancer. We suggest that miR‐10b has the potential to be used as non‐invasive breast cancer biomarker for diagnosis and prognosis and could be a novel tool in breast cancer management. Furthermore, circulating miR‐10b could help in the detection of breast cancer metastasis with better performance achieved on measuring it simultaneously with serum sE‐cadherin. Although, further studies with a large cohort of patients are warranted.
Databáze: OpenAIRE
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