A Robust Gene Expression Prognostic Signature for Overall Survival in High-Grade Serous Ovarian Cancer
| Autor: | Jian-Hua Mao, Yue Zhao, Pin Wang, Shao-Min Yang, Guang-Wu Xiong, Bo Hang, Antoine M. Snijders, Yu-Lan Jin, Xiao-Wei Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Article Subject Clinical Sciences Oncology and Carcinogenesis lcsh:RC254-282 Transcriptome 03 medical and health sciences 0302 clinical medicine Text mining Rare Diseases Internal medicine Gene expression Serous ovarian cancer medicine Genetics Genetic Testing Gene Epithelial cell differentiation Cancer screening and diagnosis Integrative bioinformatics business.industry Proportional hazards model Prevention Human Genome lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Ovarian Cancer Detection 030104 developmental biology Good Health and Well Being Medical Microbiology 030220 oncology & carcinogenesis business Research Article Biotechnology 4.2 Evaluation of markers and technologies |
| Zdroj: | Journal of oncology, vol 2019 Journal of Oncology Journal of Oncology, Vol 2019 (2019) |
| Popis: | The objective of this research was to develop a robust gene expression-based prognostic signature and scoring system for predicting overall survival (OS) of patients with high-grade serous ovarian cancer (HGSOC). Transcriptomic data of HGSOC patients were obtained from six independent studies in the NCBI GEO database. Genes significantly deregulated and associated with OS in HGSOCs were selected using GEO2R and Kaplan–Meier analysis with log-rank testing, respectively. Enrichment analysis for biological processes and pathways was performed using Gene Ontology analysis. A resampling/cross-validation method with Cox regression analysis was used to identify a novel gene expression-based signature associated with OS, and a prognostic scoring system was developed and further validated in nine independent HGSOC datasets. We first identified 488 significantly deregulated genes in HGSOC patients, of which 232 were found to be significantly associated with their OS. These genes were significantly enriched for cell cycle division, epithelial cell differentiation, p53 signaling pathway, vasculature development, and other processes. A novel 11-gene prognostic signature was identified and a prognostic scoring system was developed, which robustly predicted OS in HGSOC patients in 100 sampling test sets. The scoring system was further validated successfully in nine additional HGSOC public datasets. In conclusion, our integrative bioinformatics study combining transcriptomic and clinical data established an 11-gene prognostic signature for robust and reproducible prediction of OS in HGSOC patients. This signature could be of clinical value for guiding therapeutic selection and individualized treatment. |
| Databáze: | OpenAIRE |
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