Determination of the Ratio of the Decantation Time and the Separation of Components in Lipoaspirate
| Autor: | Roberto Montes de Oca, Gabriel A. Mecott, Mauricio M. García-Pérez, Adrian González-Martínez, José J Pérez-Trujillo, Hernán Chacón-Martínez, Ruben Gerardo Cueto-Ramos |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Annals of Plastic Surgery. 85:e7-e11 |
| ISSN: | 1536-3708 0148-7043 |
| DOI: | 10.1097/sap.0000000000002261 |
| Popis: | Decantation of the lipoaspirate is one of the most common techniques used to prepare the fat graft. The aim of the study was to determine the ideal time of decantation that provides the best separation of the components without compromising the viability of the adipocytes. Methods Thirty milliliters of fat were obtained from 11 healthy adults and decanted at room temperature for 0, 30, and 60 minutes. After decantation, the infiltration liquid and the remnant fat were measured with a volumetric pipette. Once the solution was removed, the remnant fat was centrifuged at 3000 rpm for 5 minutes to separate any residual solution, to measure the amount of actual fat obtained at that time point. Viability was determined with trypan blue staining for all the samples. Results After decantation, 9.4 ± 0.79 mL of fat was obtained at time 0, whereas 7.7 ± 1.56 mL was obtained at 30 minutes and 6.9 ± 0.92 mL at 60 minutes. Actual fat volume was 6.6 ± 1.56 mL, 5.5 ± 1.39, and 5.26 ± 1.3 mL, respectively. Viability at time 0 was 73.33 ± 0.06%, 72.57 ± 0.1% at 30 minutes, and 59.3 ± 0.09% at 60 minutes (P = 0.004). Results The fat grafting, processed by decantation, will have the best performance within a period of 30 minutes after harvesting, where the best rate of viability and separation of components will be achieved. |
| Databáze: | OpenAIRE |
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