Functionalization of a Few-Layer Antimonene with Oligonucleotides for DNA Sensing
| Autor: | Iñigo Torres, Juan Jose Palacios, Encarnación Lorenzo, Carlos Busó-Rogero, Jesús Solera, Félix Pariente, Zahra Razavifar, Félix Zamora, Cristina Gutiérrez-Sánchez, Tania García-Mendiola, Carlos Gibaja |
|---|---|
| Přispěvatelé: | UAM. Departamento de Bioquímica, UAM. Departamento de Física de la Materia Condensada, UAM. Departamento de Química Analítica y Análisis Instrumental, UAM. Departamento de Química Inorgánica |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Biblos-e Archivo. Repositorio Institucional de la UAM instname Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia Biblos-e Archivo: Repositorio Institucional de la UAM Universidad Autónoma de Madrid |
| ISSN: | 2574-0970 |
| Popis: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Nano Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/abs/10.1021/acsanm.0c00335 Antimonene, a novel group 15 two-dimensional material, is functionalized with an oligonucleotide as a first step to DNA sensor development. The functionalization process leads to a few-layer antimonene modified with DNA that after deposition on gold screen-printed electrodes gives a simple and efficient DNA electrochemical sensing platform. We provide theoretical and experimental data of the DNA–antimonene interaction, confirming that oligonucleotides interact noncovalently but strongly with antimonene. The potential utility of this antimonene-based sensing device is assessed using, as a case of study, a sequence from the BRCA1 gene as the target DNA. The selectivity of the device allows not only recognition of a specific DNA sequence but also detection of a mutation in this gene associated with breast cancer, directly in clinical samples The Ministerio de Ciencia Innovación y Universidades (Grants CTQ2017-84309-C2-1-R, MAT2016-77608-C3-1-P, PCI2018-093081, JTC2017/2D-Sb&Ge, and FIS2016-80434-P), Generalitat Valenciana (Grant APOSTD/2017/010), and CAM (Grants TransNANOAVANSENS and 2017-T1/BIO-5435) are gratefully acknowledged. We also acknowledge the María de Maeztu Programme for Units of Excellence in R&D (MDM-2014-0377), the Fundación Ramón Areces, and the computer resources and assistance provided by the Centro de Computación Científica of the Universidad Autónoma de Madrid |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|