Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends
| Autor: | Zhao-Rong Lun, Francisco J. Ayala, Jianhua Gu, Ziyin Li, Qing Zhou |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell division Cell Trypanosoma brucei brucei Aurora B kinase Protozoan Proteins cytokinesis Biology Septin 03 medical and health sciences medicine Trypanosoma brucei Phosphorylation Anaphase Cytokinesis Multidisciplinary 030102 biochemistry & molecular biology Polo-like kinase backup cytokinesis Cell cycle Biological Sciences Cell biology Vector-Borne Diseases Midbody 030104 developmental biology medicine.anatomical_structure Infectious Diseases biological phenomena cell phenomena and immunity Cell Division Subcellular Fractions |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 113, iss 12 Zhou, Q; Gu, J; Lun, ZR; Ayala, FJ; & Li, Z. (2016). Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends. Proceedings of the National Academy of Sciences of the United States of America, 113(12), 3287-3292. doi: 10.1073/pnas.1601596113. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2j02m7jw |
| Popis: | Cytokinesis in Trypanosoma brucei, an early branching protozoan, occurs along its longitudinal axis uni-directionally from the anterior tip of the new flagellum attachment zone filament toward the cell's posterior end. However, the underlying mechanisms remain elusive. Here we report that cytokinesis in T. brucei is regulated by a concerted action of Polo-like kinase, Aurora B kinase, and a trypanosome- specific protein CIF1. Phosphorylation of CIF1 by Polo-like kinase targets it to the anterior tip of the new flagellum attachment zone filament, where it subsequently recruits Aurora B kinase to initiate cytokinesis. Consistent with its role, CIF1 depletion inhibits cytokinesis initiation from the anterior end of the cell, but, surprisingly, triggers cytokinesis initiation from the posterior end of the cell, suggesting the activation of an alternative cytokinesis from the opposite cell end. Our results reveal the mechanistic roles of CIF1 and Polo-like kinase in cytokinesis initiation and elucidate the mechanism underlying the recruitment of Aurora B kinase to the cytokinesis initiation site at late anaphase. These findings also delineate a signaling cascade controlling cytokinesis initiation from the anterior end of the cell and uncover a backup cytokinesis that is initiated from the posterior end of the cell when the typical anterior-to-posterior cytokinesis is compromised. |
| Databáze: | OpenAIRE |
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