IMPACT OF CORTISOL ON alpha-ACTIN CONTENT IN VASCULAR SMOOTH MUSCLE CELLS OF FETAL SHEEP
| Autor: | Giuseppe Simonetta, Maria A Matuszek, Eugenie R. Lumbers, Karen J. Gibson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Hydrocortisone Physiology medicine.medical_treatment Blotting Western Blood Pressure Gestational Age Biology Muscle Smooth Vascular Pregnancy Physiology (medical) Internal medicine medicine.artery medicine Animals Saline Pharmacology Fetus Aorta Sheep Proteins DNA Actins Endocrinology Hydrocortisone sodium succinate Gestation Electrophoresis Polyacrylamide Gel Female Blood Gas Analysis Densitometry Glucocorticoid medicine.drug |
| Zdroj: | Clinical and Experimental Pharmacology and Physiology. 33:197-203 |
| ISSN: | 1440-1681 0305-1870 |
| DOI: | 10.1111/j.1440-1681.2006.04346.x |
| Popis: | SUMMARY 1 The effects of gestation on a-actin levels in vascular smooth muscle aortae were studied in 31 fetal sheep, aged 66–144 days (term = 150 days). Aortae were collected post-mortem. 2 Aortae, carotid and femoral arteries from two groups of chronically catheterized fetal sheep (110–114 days) were also examined. One group was infused with cortisol (n = 6; hydrocortisone sodium succinate, total dose 16.8 mg in 48 h) and the control group received saline (0.15 mol/L, 0.33 mL/h, n = 7). 3 Vascular homogenate protein was separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and western transfer. a-Actin was identified using a monoclonal mouse anti-a actin antibody and standardized against tissue protein and DNA content. 4 Between 60 and 144 days gestation, there was an exponential increase in the a-actin content of vascular smooth muscle cells from fetal sheep aorta (P |
| Databáze: | OpenAIRE |
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