Application of a novel method for optimization of bioemulsan production in a miniaturized bioreactor
| Autor: | P. Chamanrokh, Mahta Mazaheri, Hamid Rashedi, S. Jokari, Fatemeh Yazdian, Gh. Amoabediny, M. Ghavami, M. Rezvani |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Environmental Engineering Materials science Bioengineering Bacterial growth Hemolysis Agar plate Laboratory flask Bioreactors Bioreactor Animals Surface Tension Biomass Acinetobacter calcoaceticus Waste Management and Disposal Miniaturization Sheep Chromatography biology Renewable Energy Sustainability and the Environment Polysaccharides Bacterial General Medicine equipment and supplies biology.organism_classification Oxygen Petroleum Volume (thermodynamics) Emulsions Biotechnology |
| Zdroj: | Bioresource Technology. 101:9758-9764 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2010.07.009 |
| Popis: | A novel and economical method was used to optimize bacterial growth and bioemulsan production. Acinetobacter calcoaceticus PTCC 1641 was applied in an innovated synthetic medium comprised whey and mineral salt medium (MSM) in ratio of 1:1 in a shaken flask bioreactor. The optimum inoculum size, shaking frequency rate and filling volume (V(L)) using ventilation flasks were investigated that resulted in 5 vol.%, 300 rpm and 15 ml), respectively. The surface tension decreased around 48% during bioemulsan production. Due to determine bioemulsan properties, the reliability of blood agar method was confirmed as well. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect