Environmental safety and mode of action of a novel curcumin-based photolarvicide
| Autor: | Larissa Marila de Souza, Vanderlei Salvador Bagnato, Francine Perri Venturini, Natalia Mayumi Inada, Matheus Garbuio, Jaqueline Pérola Souza, Cristina Kurachi, Kleber T. de Oliveira |
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| Rok vydání: | 2020 |
| Předmět: |
Insecticides
Curcumin animal structures Health Toxicology and Mutagenesis Daphnia magna Mosquito Vectors Aedes aegypti 010501 environmental sciences Pharmacology 01 natural sciences TERAPIA FOTODINÂMICA Aedes Animals Environmental Chemistry Ecotoxicology Mode of action Larvicide 0105 earth and related environmental sciences biology Zika Virus Infection Chemistry fungi Zika Virus General Medicine biology.organism_classification Pollution Catalase Larva Toxicity biology.protein Ecotoxicity |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Aedes aegypti is the vector of important diseases like dengue, zika, chikungunya, and yellow fever. Vector control is pivotal in combating the spread of these mosquito-borne illnesses. Photoactivable larvicide curcumin obtained from Curcuma longa Linnaeus has shown high potential for Ae. aegypti larvae control. However, the toxicity of this photosensitizer (PS) might jeopardize non-target aquatic organisms. The aim of this study was to evaluate the toxicity of this PS to Daphnia magna and Danio rerio, besides assessing its mode of action through larvae biochemical and histological studies. Three PS formulations were tested: PS in ethanol+DMSO, PS in sucrose, and PS in D-mannitol. The LC50 of PS in ethanol+DMSO to D. rerio was 5.9 mg L-1, while in D. magna the solvents were extremely toxic, and LC50 was not estimated. The PS formulations in sugars were not toxic to neither of the organisms. Reactive oxygen species (ROS) were generated in D. magna exposed to 50 mg L-1 of PS in D-mannitol, and D. rerio did not elicit this kind of response. D. magna feeding rates were not affected by the PS in D-mannitol. Concerning Ae. aegypti larvae, there were changes in reduced glutathione and protein levels, while catalase activity remained stable after exposure to PS in D-mannitol and sunlight. Histological changes were observed in larvae exposed to PS in sucrose and D-mannitol, most of them irreversible and deleterious. Our results show the feasibility of this photolarvicide use in Ae. aegypti larvae control and its safety to non-target organisms. These data are crucial to this original vector control approach implementation in public health policies. |
| Databáze: | OpenAIRE |
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