Ethanol consumption produces a small increase in circulating miR-122 in healthy individuals
| Autor: | David J. Webb, James W. Dear, Noel Sharkey, A. D. Bastiaan Vliegenthart, Jame C McCrae |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Adolescent Alcohol Drinking Pharmacology Toxicology Drug overdose Gastroenterology Young Adult 03 medical and health sciences chemistry.chemical_compound Internal medicine Humans Medicine Prospective Studies Prospective cohort study Aged Liver injury Hematology Ethanol business.industry General Medicine Middle Aged medicine.disease Healthy Volunteers Confidence interval Up-Regulation Acetaminophen MicroRNAs 030104 developmental biology chemistry Toxicity Blood Alcohol Content Female business medicine.drug |
| Zdroj: | McCrae, J C, Sharkey, N, Webb, D J, Vliegenthart, A D B & Dear, J W 2016, ' Ethanol consumption produces a small increase in circulating miR-122 in healthy individuals ', Clinical Toxicology, vol. 54, no. 1, pp. 53-5 . https://doi.org/10.3109/15563650.2015.1112015 |
| ISSN: | 1556-9519 1556-3650 |
| DOI: | 10.3109/15563650.2015.1112015 |
| Popis: | INTRODUCTION: MicroRNA 122 (miR-122) is a new circulating biomarker for liver injury, which increases earlier than conventional markers in patients with acetaminophen hepatotoxicity. However, as co-ingestion of ethanol is common with drug overdose, a confounding effect of acute ethanol consumption on serum miR-122 must be examined.METHODS: Blood was collected from healthy volunteers before and after recreational consumption of ethanol. Routine biochemistry and haematology measurements were performed, and serum miR-122 was measured by qPCR. The primary outcome was the difference in serum miR-122 with ethanol consumption.RESULTS: We recruited 18 participants (72% male). Their mean serum ethanol concentration was 113 mg/dl (95% confidence interval [CI] 91-135 mg/dl) after consuming ethanol. Serum miR-122 increased from a mean of 71.3 million (95% CI 29.3-113.2 million) to 139.1 million (95% CI 62.6-215.7 million) copies/ml (2.2-fold increase). There was no significant difference in serum alanine aminotransferase activity before and after ethanol consumption.CONCLUSION: miR-122 increased with moderate ethanol consumption, but the fold change was modest. As increases with acetaminophen toxicity are 100- to 10 000-fold, moderate ethanol intoxication is unlikely to confound the use of this biomarker of hepatotoxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|