The N93D mutation of the human T-cell leukemia virus type 1 envelope glycoprotein found in symptomatic patients enhances neuropilin-1 b1 domain binding
| Autor: | Kaori Wakamatsu, Taiichi Sakamoto, Toshiyuki Tanaka, Isao Hamaguchi, Chinatsu Ohshima, Hideki Kusunoki |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Human T-lymphotropic virus 1 Binding Sites Chemistry Retroviridae Proteins Oncogenic Mutant Mutation Missense Biophysics Gene Products env Isothermal titration calorimetry Peptide HTLV-I Infections Biochemistry Molecular biology Neuropilin-1 Analytical Chemistry Viral Envelope Proteins Viral envelope Neuropilin 1 Humans Binding site Receptor Glycoprotein Molecular Biology Protein Binding |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1869:140708 |
| ISSN: | 1570-9639 |
| Popis: | Human T-cell leukemia virus type 1 (HTLV-1) infection of host cells is mainly mediated by interactions with the viral envelope glycoprotein surface unit (SU) and three host receptors: heparan sulfate proteoglycan, neuropilin-1 (Nrp1), and glucose transporter type 1. Residues 90–94 of SU are considered as a Nrp1 binding site, and our previous results show that an SU peptide consisting of residues 85–94 can bind directly to the Nrp1 b1 domain with a binding affinity of 7.4 μM. Therefore, the SU peptide is expected to be a good model to investigate the SU–Nrp1 interaction. Recently, the N93D mutation in the Nrp1 b1 binding region of the SU was identified in symptomatic patients with HTLV-1 infections in the Brazilian Amazon. However, it remains unclear how the SU-N93D mutation affects Nrp1 b1 binding. To elucidate the impact of the substituted Asp93 of SU on Nrp1 b1 binding, we analyzed the interaction between the SU-N93D peptide and Nrp1 b1 using isothermal titration calorimetry and nuclear magnetic resonance. The SU-N93D peptide binds directly to Nrp1 b1 with a binding affinity of 3.5 μM, which is approximately two-fold stronger than wild-type. This stronger binding is likely a result of the interaction between the substituted residue Asp93 of the N93D peptide and the four residues Trp301, Lys347, Glu348, and Thr349 of Nrp1 b1. Our results suggest that the interaction of SU Asp93 with the four residues of Nrp1 b1 renders the high affinity of the N93D mutant for Nrp1 b1 binding during HTLV-1 entry. |
| Databáze: | OpenAIRE |
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