Cloning and analysis of thespcribosomal protein operon ofBacillus subtilis: comparison with thespcoperon ofEscherichia coli
| Autor: | S. H. Moon, M. Nomura, Tina M. Henkin, L. C. Mattheakis |
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| Rok vydání: | 1989 |
| Předmět: |
DNA
Bacterial Ribosomal Proteins Sequence analysis Operon Molecular Sequence Data Restriction Mapping Bacillus subtilis Regulatory Sequences Nucleic Acid Biology medicine.disease_cause trp operon Ribosomal protein Sequence Homology Nucleic Acid Genetics medicine gal operon Amino Acid Sequence Promoter Regions Genetic Escherichia coli Base Sequence fungi Hydrogen Bonding biology.organism_classification Genes Bacterial Nucleic Acid Conformation L-arabinose operon |
| Zdroj: | Nucleic Acids Research. 17:7469-7486 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/17.18.7469 |
| Popis: | A segment of Bacillus subtilis chromosomal DNA homologous to the Escherichia coli spc ribosomal protein operon was isolated using cloned E. coli rplE (L5) DNA as a hybridization probe. DNA sequence analysis of the B. subtilis cloned DNA indicated a high degree of conservation of spc operon ribosomal protein genes between B. subtilis and E. coli. This fragment contains DNA homologous to the promoter-proximal region of the spc operon, including coding sequences for ribosomal proteins L14, L24, L5, S14, and part of S8; the organization of B. subtilis genes in this region is identical to that found in E. coli. A region homologous to the E. coli L16, L29 and S17 genes, the last genes of the S10 operon, was located upstream from the gene for L14, the first gene in the spc operon. Although the ribosomal protein coding sequences showed 40-60% amino acid identity with E. coli sequences, we failed to find sequences which would form a structure resembling the E. coli target site for the S8 translational repressor, located near the beginning of the L5 coding region in E. coli, in this region or elsewhere in the B. subtilis spc DNA. |
| Databáze: | OpenAIRE |
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