Kinetics of acute infection with Toxoplasma gondii and histopathological changes in the duodenum of rats
| Autor: | Gessilda de Alcântara Nogueira de Melo, Suellen Laís Vicentino-Vieira, João Luiz Garcia, Paulo da Silva Watanabe, Débora de Mello Gonçales Sant'Ana, Aline Rosa Trevizan, Marcelo Biondaro Góis, Eduardo José de Almeida Araújo |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Enterocyte Duodenum 030231 tropical medicine Immunology H&E stain Antibodies Protozoan Cell Count Periodic acid–Schiff stain Biology 03 medical and health sciences Random Allocation 0302 clinical medicine Intestinal mucosa medicine Animals Lymphocytes Intestinal Mucosa Rats Wistar Analysis of Variance Microvilli Toxoplasma gondii General Medicine biology.organism_classification Mucus Rats Kinetics 030104 developmental biology Infectious Diseases medicine.anatomical_structure Enterocytes Toxoplasmosis Animal Immunoglobulin G Intraepithelial lymphocyte Parasitology Goblet Cells Toxoplasma |
| Zdroj: | Experimental parasitology. 165 |
| ISSN: | 1090-2449 |
| Popis: | Toxoplasma gondii crosses the intestinal barrier to spread into the body. We investigate the intestinal wall and epithelial cells of the duodenum of rats infected with T. gondii during different time points of acute infection. Male Wistar rats, 60 days of age, were assigned into groups that were orally inoculated with 5000 sporulated oocysts T. gondii for 6 h (G6), 12 h (G12), 24 h (G24), 48 h (G48), 72 h (G72), 7 days (G7d), and 10 days (G10d). The control group (CG) received saline. The rats were killed and the duodenum was processed to obtain histological sections stained with hematoxylin and eosin, Periodic Acid Schiff, and Alcian blue (pH 2.5 and 1.0). Morphometry was performed on the layers of the intestinal wall and enterocytes, and the number of goblet cells and intraepithelial lymphocytes was counted. The data were compared by ANOVA considering 5% as level of significance. The infection provoked an increase in the width of villi and crypts; decrease in enterocyte height; increase in the smaller-diameter and reduction in the larger-diameter of the enterocytes nuclei, increased number of goblet cells secreting neutral (G6, G12 and G7d) and acidic (G7d and G10d) mucus, and increase in the number of intraepithelial lymphocytes (G48). The infected groups showed atrophy of the submucosa and muscular layers and the total wall. Acute infection with T. gondii caused morphological changes in the intestinal wall and epithelial cells of the duodenum in rats. |
| Databáze: | OpenAIRE |
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