Heparin Induces the Mobilization of Heart-Derived Multipotent Mesoangioblasts During Cardiac Surgery With Cardiopulmonary Bypass or Cardiac Catheterization
| Autor: | Masamichi Koyanagi, Yoshihiro Yamamoto, Stefanie Dimmeler, Yoshihiro Hata, Daisuke Sato, Andreas M. Zeiher, Miyuki Nakano, Chiharu Enoki, Haengnam Park, Yasushi Adachi, Hiroshi Kishimoto, Keisuke Fujitaka, Kensaku Wada, Naoki Minato, Masayoshi Iwasaki, Keita Horitani, Ichiro Shiojima |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cardiac Catheterization medicine.medical_specialty medicine.medical_treatment 030204 cardiovascular system & hematology law.invention 03 medical and health sciences 0302 clinical medicine law Internal medicine medicine Cardiopulmonary bypass Humans Heart Atria Progenitor cell Coronary sinus Aged Cardiac catheterization Aged 80 and over Cardiopulmonary Bypass Heparin Hepatocyte Growth Factor business.industry Mesenchymal Stem Cells General Medicine Middle Aged Cardiac surgery 030104 developmental biology Cardiology Female Hepatocyte growth factor Cardiology and Cardiovascular Medicine business Blood sampling medicine.drug |
| Zdroj: | Circulation Journal. 82:1459-1465 |
| ISSN: | 1347-4820 1346-9843 |
| Popis: | BACKGROUND We previously identified circulating mesoangioblasts (cMABs), a subset of mesenchymal stem cells that express cardiac mesodermal markers, in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). We also found that hepatocyte growth factor (HGF) is upregulated during cardiac surgery with CPB in humans, and induces MAB-like cell mobilization in rodents. These results strongly suggest that heparin induced MAB mobilization via HGF upregulation. Here, we tested this hypothesis in patients undergoing cardiac surgery or cardiac catheterization. We also examined whether human cMABs are derived from the heart.Methods and Results:Plasma HGF levels were determined by ELISA. Mononuclear cells isolated from blood samples were cultured on fibronectin-coated dishes, and outgrowing cMAB colonies were counted. We first confirmed that HGF upregulation and cMAB mobilization were observed before the start of CPB, excluding the possibility that CPB is the primary inducer of cMAB mobilization. We then examined patients undergoing cardiac catheterization and found that heparin significantly increased plasma HGF levels and the number of cMAB colonies in a dose-dependent manner. The results of simultaneous blood sampling from the aortic sinus, coronary sinus, and right atrium were consistent with the notion that human cMABs are derived from the heart. CONCLUSIONS Human cMABs are mobilized by heparin injection during cardiac surgery or cardiac catheterization, presumably via HGF upregulation. |
| Databáze: | OpenAIRE |
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