Identification of a novel rickettsial infection in a patient diagnosed with murine typhus
| Autor: | John B. Sacci, Martin E. Schriefer, Abdu F. Azad, J S Dumler, M G Bullen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) animal structures Molecular Sequence Data Murine typhus DNA Ribosomal Polymerase Chain Reaction Microbiology Diagnosis Differential Mice Species Specificity Rickettsia typhi medicine Animals Humans Rickettsia Aged DNA Primers Aged 80 and over Antigens Bacterial Base Sequence biology Rickettsia Infections Typhus Endemic Flea-Borne bacterial infections and mycoses biology.organism_classification medicine.disease Virology Rickettsia felis Spotted fever Rickettsiosis bacteria Female Rickettsiales Typhus Research Article |
| Zdroj: | Journal of Clinical Microbiology. 32:949-954 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.32.4.949-954.1994 |
| Popis: | Identification of ELB agent-infected fleas and rodents within several foci of murine typhus in the United States has prompted a retrospective investigation for this agent among human murine typhus patients. This agent is a recently described rickettsia which is indistinguishable from Rickettsia typhi with currently available serologic reagents. Molecular analysis of the 17-kDa antigen gene and the citrate synthase gene has discriminated this bacterium from other typhus group and spotted fever group rickettsiae. Current sequencing of its 16S ribosomal DNA gene indicates a homology of 98.5% with R. typhi and 99.5% with R. rickettsii. Through a combination of restriction fragment length polymorphism and Southern hybridization analysis of rickettsia-specific PCR products, one of five tested patient blood samples was shown to be infected with ELB while R. typhi infections were confirmed in the remaining samples. This is the first reported observation of a human infection by the ELB agent and underscores the utility of PCR-facilitated diagnosis and discrimination of these closely related rickettsial infections. |
| Databáze: | OpenAIRE |
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