Analysis of immunoglobulin and T-cell receptor gene rearrangements in human fetal bone marrow B lineage cells
| Autor: | T W, LeBien, R L, Elstrom, M, Moseley, J H, Kersey, F, Griesinger |
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| Rok vydání: | 1990 |
| Předmět: |
Gene Rearrangement
B-Lymphocytes Genes Immunoglobulin Immunology Receptors Antigen T-Cell Bone Marrow Cells Receptors Antigen T-Cell gamma-delta Cell Biology Hematology Gene Rearrangement T-Lymphocyte Biochemistry Cell Line Blotting Southern hemic and lymphatic diseases Humans Immunoglobulin Heavy Chains |
| Zdroj: | Blood. 76:1196-1200 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Fetal bone marrow B lineage cells representing multiple stages of B cell development were isolated by two-color cell sorting and analyzed for immunoglobulin H and T-cell receptor (TCR) gamma and delta gene rearrangements. Analysis of CD10+/surface mu- cells using a JH probe revealed a high frequency of rearrangements; some of these rearrangements used the 3′ D region gene DQ52. Analysis of CD10+/surface mu- cells revealed no detectable TCR-gamma or -delta rearrangements, nor were TCR-delta rearrangements detected in CD10+/surface mu+ cells, despite the limited repertoire of these genes. These observations are surprising given the high frequency of TCR delta/gamma rearrangements in B cell precursor acute lymphoblastic leukemia, and identify a potential difference in patterns of gene rearrangement that distinguish normal and leukemic B cell precursors. |
| Databáze: | OpenAIRE |
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