Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes
| Autor: | Gong Cheng, Jianying Liu, Jingjun Qiu, Xiaojing Pang, Penghua Wang, Senyan Du, Yang Liu, Kaixiao Nie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male viruses 030106 microbiology Immunology Biology Dengue virus Viral Nonstructural Proteins Active immunization medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Arbovirus Virus Article Cell Line Flavivirus Infections 03 medical and health sciences Mice Genetics medicine Animals Humans Vector (molecular biology) Receptors Interferon Encephalitis Virus Japanese Flavivirus Virion virus diseases Cell Biology Japanese encephalitis biochemical phenomena metabolism and nutrition Dengue Virus medicine.disease biology.organism_classification Virology 3. Good health Specific Pathogen-Free Organisms Mice Inbred C57BL 030104 developmental biology Culicidae Drosophila Female |
| Zdroj: | Nature microbiology |
| ISSN: | 2058-5276 |
| Popis: | Summary The arbovirus life cycle involves viral transfer between a vertebrate host and an arthropod vector, and acquisition of virus from an infected mammalian host by a vector is an essential step in this process. Here, we report that flavivirus nonstructural protein-1 (NS1), which is abundantly secreted into the serum of an infected host, plays a critical role in flavivirus acquisition by mosquitoes. The presence of dengue virus (DENV) and Japanese encephalitis virus (JEV) NS1s in the blood of infected interferon alpha and gamma receptor-deficient mice (AG6) facilitated virus acquisition by their native mosquito vectors because the protein enabled the virus to overcome the immune barrier of the mosquito midgut. Active immunization of AG6 mice with a modified DENV NS1 reduced DENV acquisition by mosquitoes and protected mice against a lethal DENV challenge, suggesting that immunization with NS1 could reduce the number of virus-carrying mosquitoes as well as the incidence of flaviviral diseases. Our study demonstrates that flaviviruses utilize NS1 proteins produced during their vertebrate phases to enhance their acquisition by vectors, which might be a result of flavivirus evolution to adapt to multiple host environments. |
| Databáze: | OpenAIRE |
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