Sp1 elements inSULT2B1bpromoter and 5′-untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition
| Autor: | Charles A. Strott, Yuko Higashi, Chu Luu, Young C. Lee, Chikara Shimizu |
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| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Transcriptional Activation Five prime untranslated region Sp1 Transcription Factor Molecular Sequence Data Biophysics Sulfonation Biology Response Elements Chromatin structure Biochemistry Genes Reporter Structural Biology Cis-element Genetics medicine Humans Coding region Amino Acid Sequence RNA Messenger Luciferases Promoter Regions Genetic Molecular Biology Trans-factor Cell Line Transformed Reporter gene HDAC11 HDAC10 Cell Biology Sp2 Transcription Factor HDAC4 Molecular biology DNA-Binding Proteins Histone Deacetylase Inhibitors Isoenzymes Trichostatin A SULT2B1 promoter Histone deacetylase Sulfotransferases Transcription Initiation Site 5' Untranslated Regions Transcription Factors medicine.drug |
| Zdroj: | FEBS Letters. 579:3639-3645 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2005.05.041 |
| Popis: | The steroid/sterol sulfotransferase gene (SULT2B1) encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human SULT2B1 gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5′-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibition. |
| Databáze: | OpenAIRE |
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