First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)
| Autor: | Jun Zou, Christopher J. Secombes, Steve Bird, Suman Mitra, Astrid M. van der Sar, Difei Wang, Eveline M. Weerdenburg, Sohye Yoon, Ayham Alnabulsi, Cathy A. Wyse |
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| Přispěvatelé: | Medical Microbiology and Infection Prevention, CCA - Immuno-pathogenesis |
| Rok vydání: | 2015 |
| Předmět: |
CD4-Positive T-Lymphocytes
Models Molecular Molecular Sequence Data Danio Mycobacterium Infections Nontuberculous lcsh:Medicine Adaptive Immunity Antibodies Immune system Antigen Animals Humans Amino Acid Sequence RNA Messenger lcsh:Science Th1-Th2 Balance Zebrafish Phylogeny ZAP-70 Protein-Tyrosine Kinase Multidisciplinary biology T-cell receptor lcsh:R Histocompatibility Antigens Class II Correction Zebrafish Proteins biology.organism_classification Acquired immune system Molecular biology Genetic Loci Polyclonal antibodies Mycobacterium marinum biology.protein Cytokines lcsh:Q gamma-Globulins Antibody Sequence Alignment Research Article |
| Zdroj: | Yoon, S, Mitra, S, Wyse, C, Alnabulsi, A, Zou, J, Weerdenburg, E M, van der Sar, A M, Wang, D F, Secombes, C J & Bird, S 2015, ' First Demonstration of Antigen Induced Cytokine Expression by CD4-1(+) Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio) ', PLoS ONE, vol. 10, no. 6, e0126378 . https://doi.org/10.1371/journal.pone.0126378 PLoS ONE, Vol 10, Iss 6, p e0126378 (2015) PLoS ONE PLoS ONE, 10(6):e0126378. Public Library of Science |
| ISSN: | 1932-6203 |
| Popis: | Adaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1+ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A polyclonal antibody to zfCD4-1 was developed and used with an antibody to ZAP70 and revealed double positive cells by immunohistochemistry, and in the Mycobacterium marinum disease model CD4-1+ cells were apparent surrounding the granulomas typical of the infection. Next a prime-boost experiment, using human gamma globulin as antigen, was performed and revealed for the first time in fish that zfCD4-1+ lymphocytes increase the expression of cytokines and master transcription factors relevant to Th1/Th2-type responses as a consequence of boosting with specific antigen. |
| Databáze: | OpenAIRE |
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