Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor
| Autor: | Tsao-Jen Lin, Mon-Fu Chung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Optical fiber
medicine.drug_class Analytical chemistry Monoclonal antibody lcsh:Chemical technology biosensor Biochemistry Full Research Paper Analytical Chemistry law.invention Ion Metal localized surface plasmon resonance law medicine lcsh:TP1-1185 Electrical and Electronic Engineering Surface plasmon resonance Instrumentation lead-chelate complex lead-chelate complex. antibody Detection limit gold nanoparticle monoclonal antibody Chemistry Atomic and Molecular Physics and Optics visual_art Monoclonal visual_art.visual_art_medium Biosensor |
| Zdroj: | Sensors (Basel, Switzerland) Sensors; Volume 8; Issue 1; Pages: 582-593 Sensors, Vol 8, Iss 1, Pp 582-593 (2008) |
| ISSN: | 1424-8220 |
| Popis: | A novel reflection-based localized surface plasmon resonance (LSPR) fiber-optic probe has been developed to determine the heavy metal lead ion concentration. Monoclonal antibody as the detecting probe containing massive amino groups to capture Pb(II)-chelate complexes was immobilized onto gold nanoparticle-modified optical fiber (NMAuOF). The optimal immobilizing conditions of monoclonal antibody on to the NMAuOF are 189 μg/mL in pH7.4 PBS for 2 h at 25°C. The absorbability of the functionalized NMAuOF sensor increases to 12.2 % upon changing the Pb(II)-EDTA level from 10 to 100 ppb with a detection limit of 0.27 ppb. The sensor retains 92.7 % of its original activity and gives reproducible results after storage in 5% D-( )-Trehalose dehydrate solution at 4°C for 35 days. In conclusion, the monoclonal antibody-functionalized NMAuOF sensor shows a promising result for determining the concentration of Pb(II) with high sensitivity. |
| Databáze: | OpenAIRE |
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