KLF13 induces apoptotic cell clearance in Penaeus vannamei as an essential part of shrimp innate immune response to pathogens
| Autor: | Defu Yao, Shiyuan Bao, Hongyu Ma, Tran Ngoc Tuan, Chuchu Zhang, Yongzhen Zhao, Jude Juventus Aweya, Yueling Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Mammals
Innate immune system biology Base Sequence Vibrio parahaemolyticus Immunology White spot syndrome Apoptosis biology.organism_classification Immunity Innate Shrimp Microbiology Arthropod Proteins Apoptotic cell clearance Open reading frame Immune system White spot syndrome virus 1 Gene Expression Regulation Penaeidae Animals Amino Acid Sequence Transcription factor Sequence Alignment Phylogeny Developmental Biology |
| Zdroj: | Developmental and comparative immunology. 126 |
| ISSN: | 1879-0089 |
| Popis: | Although, in mammals, the Kruppel-like transcription factor 13 (KLF13) plays an essential role in cell proliferation, survival, differentiation, apoptosis, tumorigenesis, immune regulation, and inflammation, its role in penaeid shrimp is unclear. In the current study, we characterized a KLF13 homolog in Penaeus vannamei (PvKLF13), with full-length cDNA of 1677 bp and 1068 bp open reading frame (ORF) encoding a putative protein of 355 amino acids, which contains three ZnF_C2H2 domains. Sequence and phylogenetic analysis revealed that PvKLF13 shares a close evolutionary relationship with KLF13 from invertebrates. Transcript levels of PvKLF13 were ubiquitously expressed in shrimp and induced in hemocytes upon challenge with Vibrio parahaemolyticus, Streptococcus iniae, and white spot syndrome virus (WSSV), suggesting the involvement of PvKLF13 in shrimp immune response to pathogens. Besides, knockdown of PvKLF13 decreased hemocytes apoptosis in terms of increased expression of pro-survival PvBcl-2, but decreased expression of pro-apoptotic PvBax and PvCytochrome C, coupled with high PvCaspase3/7 activity, especially upon V. parahaemolyticus challenge. The findings here indicate the involvement of PvKLF13 in apoptotic cell clearance as an essential part of shrimp innate immune response to pathogens. |
| Databáze: | OpenAIRE |
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