Understanding gold toxicity in aerobically-grown Escherichia coli
Autor: | Claudia M. Muñoz-Villagrán, D. Valenzuela-Bezanilla, M. Castro, Felipe A. Arenas, F. Contreras, Roberto Luraschi, Jaime Andrés Rivas-Pardo, Maximiliano Figueroa, C. Reinoso, Claudio C. Vásquez, Fabián A. Cornejo |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Anaerobic Antioxidant medicine.medical_treatment Resistance Metal Nanoparticles 02 engineering and technology Oxidative phosphorylation medicine.disease_cause Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound medicine Escherichia coli lcsh:QH301-705.5 biology Toxicity Superoxide E. coli Aerobic 021001 nanoscience & nanotechnology Oxidative Stress 030104 developmental biology lcsh:Biology (General) chemistry Biochemistry Gold(III) Catalase biology.protein Gold 0210 nano-technology Oxidation-Reduction Oxidative stress Research Article |
Zdroj: | Biological Research v.53 2020 SciELO Chile CONICYT Chile instacron:CONICYT Biological Research Biological Research, Vol 53, Iss 1, Pp 1-9 (2020) |
Popis: | Background There is an emerging field to put into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organism are still unknown. Here we aimed to investigate and analyze Au3+ toxicity through a combination of biochemical and molecular approaches. Results We found that Au3+ triggers a major oxidative unbalance in Escherichia coli, characterized by decreased intracellular thiol levels, increased superoxide concentration, as well as by an augmented production of the antioxidant enzymes superoxide dismutase and catalase. Because ROS production is, in some cases, associated with metal reduction and the concomitant generation of gold-containing nanostructures (AuNS), this possibility was evaluated in vivo and in vitro. Conclusions Au3+ is toxic for E. coli because it triggers an unbalance of the bacterium’s oxidative status. This was demonstrated by using oxidative stress dyes and antioxidant chemicals as well as gene reporters, RSH concentrations and AuNS generation. |
Databáze: | OpenAIRE |
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