Viral genome integration of canine papillomavirus 16
| Autor: | Peter Rowland, Monica Britton, Michelle Marie Mader, Hang Yuan, Jennifer Luff, Joseph Fass |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Skin Neoplasms Carcinogenesis viruses Virus Integration Integration Genome Viral medicine.disease_cause Genome DNA sequencing Translocation Genetic Article lcsh:Infectious and parasitic diseases Canine 03 medical and health sciences 0302 clinical medicine Dogs Virology Squamous cell carcinoma medicine Primer walking Animals lcsh:RC109-216 030212 general & internal medicine Dog Diseases Gene Papillomaviridae Sequence Deletion business.industry Papillomavirus Infections Cancer Papillomavirus medicine.disease Viral plaque Transformation (genetics) Infectious Diseases 030220 oncology & carcinogenesis DNA Viral Carcinoma Squamous Cell business |
| Zdroj: | Papillomavirus Research Papillomavirus Research, Vol 7, Iss, Pp 88-96 (2019) |
| ISSN: | 2405-8521 |
| Popis: | Papillomaviruses infect humans and animals, most often causing benign proliferations on skin or mucosal surfaces. Rarely, these infections persist and progress to cancer. In humans, this transformation most often occurs with high-risk papillomaviruses, where viral integration is a critical event in carcinogenesis. The first aim of this study was to sequence the viral genome of canine papillomavirus (CPV) 16 from a pigmented viral plaque that progressed to metastatic squamous cell carcinoma in a dog. The second aim was to characterize multiple viral genomic deletions and translocations as well as host integration sites. The full viral genome was identified using a combination of PCR and high throughput sequencing. CPV16 is most closely related to chipapillomaviruses CPV4, CPV9, and CPV12 and we propose CPV16 be classified as a chipapillomavirus. Assembly of the full viral genome enabled identification of deletion of portions of the E1 and E2/E4 genes and two viral translocations within the squamous cell carcinoma. Genome walking was performed which identified four sites of viral integration into the host genome. This is the first description of integration of a canine papillomavirus into the host genome, raising the possibility that CPV16 may be a potential canine high-risk papillomavirus type. Keywords: Canine, Papillomavirus, Squamous cell carcinoma, Integration, Carcinogenesis |
| Databáze: | OpenAIRE |
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