Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
| Autor: | Thalles de Freitas Castro, André Talvani, Frank Silva Bezerra, Leandro da Silva Cândido, Aline Maria dos Santos, Walter A. Zin, Guilherme de Paula Costa, Laisy Cristina de Paula, Natália Alves de Matos, Sílvia Dantas Cangussú |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Aging Article Subject medicine.medical_treatment Lung injury Protein oxidation Biochemistry Parenchyma Tidal Volume medicine Animals Rats Wistar Mechanical ventilation Lung QH573-671 medicine.diagnostic_test business.industry Lung Injury Pneumonia Cell Biology General Medicine respiratory system Respiration Artificial Rats respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Anesthesia Breathing Cytokines Arterial blood Cytology business Oxidation-Reduction Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However, MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox imbalance might also be associated with pulmonary inflammatory status, which has not been so far described. We tested it by delivering different tidal volumes to normal lungs undergoing MV. Healthy Wistar rats were divided into spontaneously breathing animals (control group, CG), and rats were submitted to MV (controlled ventilation mode) with tidal volumes of 4 mL/kg (MVG4), 8 mL/kg (MVG8), or 12 mL/kg (MVG12), zero end-expiratory pressure (ZEEP), and normoxia ( Fi O 2 = 21 % ) for 1 hour. After ventilation and euthanasia, arterial blood, bronchoalveolar lavage fluid (BALF), and lungs were collected for subsequent analysis. MVG12 presented lower PaCO2 and bicarbonate content in the arterial blood than CG, MVG4, and MVG8. Neutrophil influx in BALF and MPO activity in lung tissue homogenate were significantly higher in MVG12 than in CG. The levels of CCL5, TNF-α, IL-1, and IL-6 in lung tissue homogenate were higher in MVG12 than in CG and MVG4. In the lung parenchyma, the lipid peroxidation was more important in MVG12 than in CG, MVG4, and MVG8, while there was more protein oxidation in MVG12 than in CG and MVG4. The stereological analysis confirmed the histological pulmonary changes in MVG12. The association of controlled mode ventilation and high tidal volume, without PEEP and normoxia, impaired pulmonary histoarchitecture and triggered redox imbalance and lung inflammation in healthy adult rats. |
| Databáze: | OpenAIRE |
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