Comparison of nasopharyngeal swab and nasopharyngeal aspiration in adults for SARS‐CoV‐2 identification using reverse transcription‐polymerase chain reaction

Autor: Ozan Muzaffer Altuntaş, Selin Ünsaler, Ozan Gökler, Suda Tekin, Ayse Okan, Ayşenur Meriç Hafiz
Přispěvatelé: Altuntaş, Muzaffer Ozan (ORCID 0000-0002-8747-7420 & YÖK ID 169795), Gökler, Ozan (ORCID 0000-0003-1621-3687 & YÖK ID 311179), Hafız, Ayşenur Meriç (ORCID 0000-0002-8003-7879 & YÖK ID 53676), Ünsaler, Selin (ORCID 0000-0001-7108-9194 & YÖK ID 167909), Okan, Ayşe, Koruk, Süda Tekin (ORCID 0000-0001-9419-8713 & YÖK ID 42146), Koç University Hospital, School of Medicine
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Adult
Male
medicine.medical_specialty
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Statistical difference
Gastroenterology
Sensitivity and Specificity
SARS‐CoV‐2
Specimen Handling
COVID-19 Testing
Internal medicine
Virology
Nasopharynx
medicine
Humans
nasopharyngeal aspiration
reverse transcription real‐time polymerase chain reaction
Research Articles
Aged
business.industry
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Reproducibility of Results
Negativity effect
Mean age
Middle Aged
nasopharyngeal swab
Reverse transcription polymerase chain reaction
Infectious Diseases
Female
business
Nasopharyngeal aspiration
Nasopharyngeal swab
Reverse transcription real-time polymerase chain reaction
Research Article
Zdroj: Journal of Medical Virology
ISSN: 1096-9071
0146-6615
Popis: We aimed to compare reverse transcription‐polymerase chain reaction (RT‐PCR) results of nasopharyngeal aspiration (NA) and nasopharyngeal swab (NS) samples in the diagnosis of coronavirus disease 2019. NS was obtained with a dacron swab and NA was performed by aspiration cannula. The sampling was performed by an otolaryngologist to ensure standardized correct sampling from the nasopharynx. RT‐PCR was performed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). The level of agreement between the result of NA and NS samples for each patient was analyzed. The Ct values were compared. Thirty‐three patients were enrolled in the study with a mean age of 56.3 years. Thirteen subjects resulted negative with both NS and NA; 20 subjects resulted positive with NA and 18 subjects resulted positive with NS. The mean values of Ct for NA samples and NS samples were 24.6 ± 5.9 and 24 ± 6.7, respectively. There was no statistical difference between Ct values of NA and NS samples (p = 0.48). RT‐PCR for SARS‐Cov2 performed with NA sample and NS sample showed a strong correlation regarding the positivity/negativity and the Ct values.
Highlights The results demonstrated that RT–PCR for SARS–Cov2 performed with NA sample and NS sample showed a strong correlation regarding the positivity and negativity and the Ct values.It is less likely to obtain an insufficient amount of material with NA than that with NS. Therefore, NA may be preferred for the second sampling, especially in patients who are difficult to be sampled with swab because of a septal deviation or patient incompatibility.
Databáze: OpenAIRE
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