Using Bifunctional Polymers Presenting Vancomycin and Fluorescein Groups To Direct Anti-Fluorescein Antibodies to Self-Assembled Monolayers Presenting d -Alanine-d -Alanine Groups
| Autor: | George M. Whitesides, Ravi S. Kane, Steven J. Metallo, R. Erik Holmlin |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Surface Properties
Stereochemistry Acrylic Resins Fluorescent Antibody Technique Biochemistry Antibodies Catalysis chemistry.chemical_compound Colloid and Surface Chemistry Molecular recognition Vancomycin Fluorescence microscope Non-covalent interactions Fluorescein Bifunctional Alanine chemistry.chemical_classification Chemistry Self-assembled monolayer Dipeptides General Chemistry Polymer Surface Plasmon Resonance Fluoresceins Combinatorial chemistry Microscopy Fluorescence |
| Zdroj: | Journal of the American Chemical Society. 125:4534-4540 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja030045a |
| Popis: | This paper describes the synthesis of bifunctional polyacrylamides containing pendant vancomycin (Van) and fluorescein groups, and the use of these polymers to direct antibodies against fluorescein to self-assembled monolayers (SAMs) presenting d-alanine-d-alanine (dAdA) groups. These polymers bind biospecifically to these SAMs via interactions between the dAdA and Van groups and serve as a molecular bridge between the anti-fluorescein antibodies and the SAM. The binding events were characterized using surface plasmon resonance spectroscopy and fluorescence microscopy. The paper demonstrates that polyvalent, biospecific, noncovalent interactions between a polymer and a surface can be used to tailor the properties of the surface in molecular recognition. It also represents a first step toward the design of polymers that direct arbitrarily chosen antibodies to the surfaces of cells. |
| Databáze: | OpenAIRE |
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