Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020
| Autor: | Brigitte Altmann, Eric Gottwald, Christoph Grün |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Life sciences
biology Cell type microfluidics Bioengineering 02 engineering and technology Computational biology Biology lcsh:Chemical technology lcsh:Chemistry 03 medical and health sciences 3D cell culture Cellular Microenvironment ddc:570 Bioreactor Chemical Engineering (miscellaneous) Cell stimulation lcsh:TP1-1185 030304 developmental biology in vitro models Data source 0303 health sciences National library Process Chemistry and Technology technology industry and agriculture cellular microenvironment 021001 nanoscience & nanotechnology equipment and supplies lcsh:QD1-999 micro-bioreactor Cell culture material scaffolds tissue engineering HTS 0210 nano-technology |
| Zdroj: | Processes, 8 (12), Art.-Nr.: 1656 Processes, Vol 8, Iss 1656, p 1656 (2020) |
| ISSN: | 2227-9717 |
| DOI: | 10.5445/ir/1000129666 |
| Popis: | Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020. |
| Databáze: | OpenAIRE |
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