Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury
| Autor: | Alain Harf, K. Koumanov, Laurent Brochard, C. Wolf, M. Alaoui-El-Azher, Christophe Delclaux, Lhousseine Touqui, H.L. Attalah, Yongzheng Wu, F. Thouron |
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| Přispěvatelé: | Physiopathologie et Thérapeutiques Respiratoires, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10, Défense innée et inflammation, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de biochimie [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Réanimation Médicale [CHU Henri Mondor - APHP] (DHU A-TVB), Université Paris-Est Créteil, Faculté de Médecine [Créteil] (UPEC-Médecine)-CHU Henri Mondor, Service de physiologie, explorations fonctionnelles [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), The authors would like to thank H. Arita (Shionogi Research laboratory, Osaka, Japan) for the generous gift of the rat sPLA2-IIA cDNA., CHU Henri Mondor-Université Paris-Est Créteil, Faculté de Médecine [Créteil] (UPEC-Médecine), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Wu, Yongzheng, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Henri Mondor [Créteil]-Université Paris-Est Créteil, Faculté de Médecine [Créteil] (UPEC-Médecine) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Male
MESH: Lung Compliance / physiology Pathology endotoxin MESH: Pulmonary Edema / physiopathology MESH: Rats Sprague-Dawley Pulmonary compliance MESH: Phospholipases A / analysis Severity of Illness Index [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract Rats Sprague-Dawley 0302 clinical medicine Pulmonary surfactant MESH: Pneumonia / complications MESH: Animals Respiratory system MESH: Pulmonary Alveoli / chemistry Lung Compliance MESH: Phospholipases A2 Respiratory Distress Syndrome 0303 health sciences alpha naphthylthiourea medicine.diagnostic_test biology respiratory system MESH: Respiratory Distress Syndrome Adult / physiopathology MESH: Group II Phospholipases A2 medicine.anatomical_structure Pseudomonas aeruginosa MESH: Pulmonary Edema / complications Pulmonary alveolus Bronchoalveolar Lavage Fluid Pulmonary and Respiratory Medicine medicine.medical_specialty MESH: Rats Pulmonary Edema Lung injury Group II Phospholipases A2 compliance Phospholipases A MESH: Pneumonia / physiopathology MESH: Pulmonary Surfactants / chemistry 03 medical and health sciences Phospholipase A2 Internal medicine MESH: Severity of Illness Index medicine Animals 030304 developmental biology MESH: Pulmonary Alveoli / physiopathology Lung business.industry Pulmonary Surfactants Pneumonia MESH: Bronchoalveolar Lavage Fluid / chemistry MESH: Phospholipases A / physiology MESH: Male Rats Pulmonary Alveoli Disease Models Animal Phospholipases A2 Bronchoalveolar lavage Endocrinology 030228 respiratory system biology.protein [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract MESH: Respiratory Distress Syndrome Adult / etiology MESH: Disease Models Animal business |
| Zdroj: | European Respiratory Journal European Respiratory Journal, European Respiratory Society, 2003, 21 (6), pp.1040-1045. ⟨10.1183/09031936.03.00093002⟩ European Respiratory Journal, 2003, 21 (6), pp.1040-1045. ⟨10.1183/09031936.03.00093002⟩ |
| ISSN: | 0903-1936 1399-3003 |
| DOI: | 10.1183/09031936.03.00093002⟩ |
| Popis: | International audience; The aim of this study was to evaluate the presence of type-II secretory phospholipase A2 (sPLA2-IIA) in alveolar space and its possible role in the destruction of surfactant in three rat models of acute lung injury. Alveolar instillation of either lipopolysaccaride or live Pseudomonas aeruginosa resulted in a significant increase in lung oedema and in a decrease in static compliance of the respiratory system together with alveolar-neutrophil influx as compared with healthy control rats. The upregulation of messenger ribonucleic acid and sPLA2-IIA by the lung was evident. This was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in bacteria-instilled rats. A negative correlation between compliance and sPLA2-IIA activity in bronchoalveolar lavage fluid was shown. By contrast, during alpha naphthylthiourea-induced injury, neither alveolar-neutrophil influx nor increase in sPLA2-IIA activity was observed. Additional experiments in rats treated with a specific inhibitor of type-II secretory phospholipase A2 activity (3 acetamine-1-benzyl-2 ethylindolyl-5 oxy; propane phosphonic acid (LY311727)) demonstrated no improvement in physiological parameters despite a biochemical effect, suggesting that its activity is only one of the multiple factors involved in the pathophysiology of lung injury. |
| Databáze: | OpenAIRE |
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