Switchable Solvent Selective Extraction of Hydrophobic Antioxidants from Synechococcus bigranulatus
| Autor: | Wim Brilman, Antonino Pollio, Davide Liberti, Giuseppe Olivieri, Paola Imbimbo, Francesco Bolinesi, Luigi D’Elia, Olga Mangoni, Daria Maria Monti |
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| Přispěvatelé: | D'Elia, L., Imbimbo, P., Liberti, D., Bolinesi, F., Pollio, A., Mangoni, O., Brilman, W., Olivieri, G., Monti, D. M. |
| Rok vydání: | 2021 |
| Předmět: |
Bio Process Engineering
antioxidant N-ethylbutylamine Antioxidant Biocompatibility General Chemical Engineering medicine.medical_treatment Fraction (chemistry) chemistry.chemical_compound medicine Environmental Chemistry Carotenoid chemistry.chemical_classification Chromatography green chemistry Renewable Energy Sustainability and the Environment microalgae Extraction (chemistry) switchable solvents food and beverages General Chemistry Zeaxanthin Solvent antioxidants chemistry Amine gas treating |
| Zdroj: | ACS Sustainable Chemistry and Engineering, 9(41), 13798-13806 ACS Sustainable Chemistry and Engineering 9 (2021) 41 |
| ISSN: | 2168-0485 |
| Popis: | Hydrophobic molecules, in particular, carotenoids, have been directly extracted from Synechococcus bigranulatus ACUF680 by means of secondary amine switchable solvent N-ethylbutylamine (EBA) without any other pretreatment. EBA was able to extract hydrophobic molecules from both fresh and frozen biomass at the same extent of the conventional procedure (about 20% of dry biomass). In particular, selective extraction of a zeaxanthin-enriched fraction (green fraction, GF) and a β-carotene-enriched fraction (orange fraction, OF) was obtained. The ratio between zeaxanthin and β-carotene was 4.4 ± 1.5 for GF, 0.07 ± 0.06 for OF, and about 1 for conventional extraction. These fractions showed in vitro antioxidant activity (IC50 values of 0.056 ± 0.013 and 0.024 ± 0.008 mg mL-1 for GF and OF, respectively) and biocompatibility on immortalized cells. Moreover, OF and GF were able to protect cells from oxidative stress, both before and after thermal treatment. Results clearly indicate that EBA is a good candidate to specifically extract β-carotene and zeaxanthin from the wet biomass of S. bigranulatus without affecting their biological activity. Skipping energy-intensive operations to break the cells and using either fresh or frozen biomass may be the driving factors to use EBA switchable solvent on an industrial scale. |
| Databáze: | OpenAIRE |
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