Gene expression profiling of the canine placenta during normal and antigestagen-induced luteolysis
| Autor: | Hubert Rehrauer, Ewa Kautz, Mariusz P. Kowalewski, Serhan Serhat Ay, Murat Findik, Marta Nowak, Alois Boos |
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| Přispěvatelé: | OMÜ |
| Rok vydání: | 2019 |
| Předmět: |
Placenta
Luteolysis 030209 endocrinology & metabolism Dog Parturition Transcriptome Luteal phase Biology Dinoprost Andrology 03 medical and health sciences Dogs 0302 clinical medicine Endocrinology Immune system Pregnancy medicine Animals Gene Regulatory Networks Progesterone reproductive and urinary physiology 030304 developmental biology 0303 health sciences Gene Expression Profiling Molecular Sequence Annotation medicine.disease Gene expression profiling medicine.anatomical_structure Gene Expression Regulation Apoptosis Female Animal Science and Zoology Progestins |
| Zdroj: | General and Comparative Endocrinology, 282 |
| ISSN: | 0016-6480 |
| Popis: | FINDIK, Murat/0000-0003-1408-2548; Kowalewski, Mariusz P./0000-0002-4565-7714; Kautz, Ewa/0000-0002-8240-1224 WOS: 000485337100004 PubMed: 31145892 The domestic dog is the only domestic animal species that does not produce steroids in the placenta and instead relies on luteal steroids throughout pregnancy. Nevertheless, the canine placenta is highly responsive to steroids, and withdrawal of progesterone (P4) affects the feto-matemal unit, initializing the parturition cascade. Similar effects can be observed during antigestagen-induced abortion. Here, aiming to provide new insights into mechanisms involved in the termination of canine pregnancy, next generation sequencing (NGS, RNA-seq) was applied. Placental transcriptomes derived from natural prepartum and antigestagen-induced abortions were analyzed and compared with fully developed mid-gestation placentas. The contrast "prepartum luteolysis over mid-gestation" revealed 1973 differentially expressed genes (DEG). Terms associated with apoptosis, impairment of vascular function and activation of signaling of several cytokines (e.g., IL-8, IL-3, TGF-beta) were over-represented at natural luteolysis. When compared with mid-term, antigestagen treatment revealed 135 highly regulated DEG that were involved in the induced luteolysis and showed similar associations with functional terms and expression patterns as during natural luteolysis. The contrast "antigestagen-induced luteolysis over prepartum luteolysis" revealed that, although similar changes occur in both conditions, they are more pronounced during natural prepartum. Among P4-regulated DEG were those related to immune system and cortisol metabolism. It appears that, besides inducing placental PGF2 alpha output, both natural and induced P4 withdrawal is associated with disruption of the feto-matemal interface, leading to impaired vascular functions, apoptosis and controlled modulation of the immune response. The time-related maturation of the feto-maternal interface needs to be considered because it may be clinically relevant. Swiss National Science Foundation (SNSF)Swiss National Science Foundation (SNSF) [31003A_182481] This work was supported by The Swiss National Science Foundation (SNSF) research Grant Number 31003A_182481 to MPK. |
| Databáze: | OpenAIRE |
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