Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis
| Autor: | Jian Chen, Hui Li, Shun-Li Hu, Jin-Yun Chen, Hong Wu, Miao-Miao Dai |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.drug_class Colon p38 mitogen-activated protein kinases Immunology Freund's Adjuvant Cmax Arthritis Pharmacology Gardenia jasminoides p38 Mitogen-Activated Protein Kinases Anti-inflammatory Rats Sprague-Dawley Pharmacokinetics Oral administration medicine Immunology and Allergy Animals Humans Iridoids Cells Cultured Cell Proliferation biology Chemistry Anti-Inflammatory Agents Non-Steroidal Synovial Membrane Interleukin Fibroblasts biology.organism_classification medicine.disease Gardenia Arthritis Experimental Rats Disease Models Animal Fruit Cytokines Phytotherapy Signal Transduction |
| Zdroj: | International immunopharmacology. 24(1) |
| ISSN: | 1878-1705 |
| Popis: | The aim of this study was to explore the anti-inflammatory effects of Geniposide (GE), an iridoid glycoside compound extracted from Gardenia jasminoides Ellis (GJ) fruit in adjuvant-induced arthritis (AA) rats and its pharmacokinetic (PK) basis. AA was induced by injecting with Freund's complete adjuvant (FCA). Male SD rats were subjected to treatment with GE (30, 60 and 120 mg/kg) from day 17 to 24 after immunization. Fibroblast-like synoviocyte (FLS) proliferation was assessed by MTT. Interleukin (IL)-1, IL-6, TNF-α and IL-10 were determined using double-sandwich enzyme-linked immunosorbent assay (ELISA). Expression of p38 mitogen-activated protein kinases (p38MAPKs) related proteins in FLS was detected by Western blotting. PK profiles were simultaneously detected by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) in AA rat plasma after oral administration of GE on day 17 after immunization. As a result, GE promoted the recovery of arthritis and inhibited the colonic inflammation damage in AA rats by decreasing the expression level of TNF-α, IL-1 and IL-6, increasing the production of IL-10 and inhibiting the expression of phospho-p38 (p-p38) related proteins in FLS. PK parameters (AUC, Cmax and t1/2) tended to be associated with dosage-related decreasing of efficacy index. |
| Databáze: | OpenAIRE |
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