RNA-Mediated Programming of Developmental Genome Rearrangements in Paramecium tetraurelia
| Autor: | Vincent Serrano, Eric Meyer, Sandra Duharcourt, Olivier Garnier |
|---|---|
| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Régulation de l'expression génétique (REG), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Genetic Vectors
Gene Dosage Biology 03 medical and health sciences 0302 clinical medicine Transformation Genetic Nuclear dimorphism Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene Silencing Transgenes Molecular Biology Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Paramecium aurelia RNA Double-Stranded Genetics Gene Rearrangement 0303 health sciences Macronucleus Base Sequence RNA Cell Biology Gene rearrangement biology.organism_classification DNA Dynamics and Chromosome Structure Paramecium tetraurelia Programmed DNA elimination Genome Protozoan 030217 neurology & neurosurgery Gene Deletion RNA Protozoan |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2004, 24 (17), pp.7370-7379. ⟨10.1128/MCB.24.17.7370-7379.2004⟩ |
| ISSN: | 0270-7306 1098-5549 |
| Popis: | The germ line genome of ciliates is extensively rearranged during development of the somatic macronucleus. Numerous sequences are eliminated, while others are amplified to a high ploidy level. In the Paramecium aurelia group of species, transformation of the maternal macronucleus with transgenes at high copy numbers can induce the deletion of homologous genes in sexual progeny, when a new macronucleus develops from the wild-type germ line. We show that this trans-nuclear effect correlates with homology-dependent silencing of maternal genes before autogamy and with the accumulation of approximately 22- to 23-nucleotide (nt) RNA molecules. The same effects are induced by feeding cells before meiosis with bacteria containing double-stranded RNA, suggesting that small interfering RNA-like molecules can target deletions. Furthermore, experimentally induced macronuclear deletions are spontaneously reproduced in subsequent sexual generations, and reintroduction of the missing gene into the variant macronucleus restores developmental amplification in sexual progeny. We discuss the possible roles of the approximately 22- to 23-nt RNAs in the targeting of deletions and the implications for the RNA-mediated genome-scanning process that is thought to determine developmentally regulated rearrangements in ciliates. |
| Databáze: | OpenAIRE |
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