Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics
| Autor: | Julian M. Jimenez, Chenwei Duan, Craig J. Goergen, Abigail Cox, Preeti M. Sivasankar, Sarah Calve |
|---|---|
| Rok vydání: | 2023 |
| Předmět: |
Fold (higher-order function)
LPN and LVN medicine.disease Article Extracellular matrix 030507 speech-language pathology & audiology 03 medical and health sciences Speech and Hearing chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Otorhinolaryngology chemistry Hyaluronidase Vocal folds Hyaluronic acid otorhinolaryngologic diseases medicine Biophysics Tonicity Dehydration 030223 otorhinolaryngology 0305 other medical science Ex vivo medicine.drug |
| Zdroj: | J Voice |
| ISSN: | 0892-1997 |
| DOI: | 10.1016/j.jvoice.2021.01.014 |
| Popis: | Summary Objectives The understanding of vocal fold hydration state, including dehydrated, euhydrated, rehydrated tissue, and how hydration affects vocal fold biomechanical properties is still evolving. Although clinical observations support the benefits of increasing vocal fold hydration after dehydrating events, more mechanistic information on the effects of vocal fold dehydration and the beneficial effects of rehydration are needed. Alterations to hyaluronic acid (HA), an important component of the vocal fold extracellular matrix, are likely to influence the biomechanical properties of vocal folds. In this study, we investigated the influence of hydration state and HA on vocal fold tissue stiffness via biomechanical testing. Study design Prospective, ex vivo study design. Methods Fresh porcine vocal folds (N = 18) were examined following sequential immersion in hypertonic (dehydration) and isotonic solutions (rehydration). In a separate experiment, vocal folds were incubated in hyaluronidase (Hyal) to remove HA. Control tissues were not exposed to any challenges. A custom micromechanical system with a microforce sensing probe was used to measure the force-displacement response. Optical strain was calculated, and ultrasound imaging was used to measure tissue cross-sectional area to obtain stress-strain curves. Results Significant increases (P ≤ 0.05) were found in tangent moduli between dehydrated and rehydrated vocal folds at strains of e = 0.15. The tangent moduli of Hyal-digested tissues significantly increased at both e = 0.15 and 0.3 (P ≤ 0.05). Conclusion Vocal fold dehydration increased tissue stiffness and rehydration reduced the stiffness. Loss of HA increased vocal fold stiffness, suggesting a potential mechanical role for HA in euhydrated vocal folds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect