Detection and localization of two constitutive NOS isoforms in bull spermatozoa
| Autor: | R. Schulz, H. Meiser |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
endocrine system Nitric Oxide Synthase Type III Blotting Western Nitric Oxide Synthase Type I Endothelial NOS Immunofluorescence Nitric Oxide Nitric oxide chemistry.chemical_compound Western blot Capacitation Enos medicine Animals Acrosome reproductive and urinary physiology Microscopy Confocal General Veterinary medicine.diagnostic_test biology urogenital system General Medicine biology.organism_classification Molecular biology Immunohistochemistry Spermatozoa Nitric oxide synthase chemistry biology.protein Cattle Nitric Oxide Synthase Sperm Capacitation |
| Zdroj: | Anatomia, histologia, embryologia. 32(6) |
| ISSN: | 0340-2096 |
| Popis: | Summary Bull spermatozoa were examined for the presence and localization of constitutive Nitric Oxide Synthase (NOS), as nitric oxide (NO) is involved in calcium-dependent capacitation. In bull spermatozoa, NO generation is enhanced by l-arginine (3 μm) and abolished by the NOS-inhibitor N-nitro-l-arginine methyl ester (l-NAME). In addition, presence of NOS in bull spermatozoa was verified by immunohistochemistry, revealing the existence of both neuronal NOS (nNOS) and endothelial NOS (eNOS) immunoreaction. These findings were confirmed by Western blot technique, showing immunoreactive bands at 161 kDa (nNOS) and 133 kDa (eNOS). Confocal laser microscopy localized nNOS related immunofluorescence at the acrosome cap of sperms and their flagellum-mainpart. This technique also identified eNOS staining spread over the spermatozoan head. In conclusion, immunohistochemistry, Western blot technique, and NO generation suggest the presence of n- and eNOS in bull spermatozoa. |
| Databáze: | OpenAIRE |
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