The structure of fucoidan from Sargassum oligocystum and radiosensitizing activity of galactofucans from some algae of genus Sargassum
| Autor: | Roza V. Usoltseva, Huynh Hoang Nhu Khanh, Svetlana P. Ermakova, Artem S. Silchenko, Tatiana N. Zvyagintseva, Dinh Thanh Trung, Cao Thi Thuy Hang, Olesya S. Malyarenko, Natalia M. Shevchenko, Pham Duc Thinh, Stanislav D. Anastyuk, Vladimir V. Isakov |
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| Rok vydání: | 2021 |
| Předmět: |
Radiation-Sensitizing Agents
02 engineering and technology Polysaccharide Biochemistry Fucose 03 medical and health sciences chemistry.chemical_compound Sulfation Algae Polysaccharides Structural Biology Cell Line Tumor Humans Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Chemistry Fucoidan Sargassum General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Galactose Cancer cell 0210 nano-technology HT29 Cells |
| Zdroj: | International Journal of Biological Macromolecules. 183:1427-1435 |
| ISSN: | 0141-8130 |
| Popis: | The aim of this study was to establish the fine structure of fucoidan from Sargassum oligocystum and to study the radiosensitizing effect of fucoidans from three algae of genus Sargassum (S. oligocystum, S. duplicatum, and S. feldmannii) with different structures. The fucoidan SoF2 from S. oligocystum was sulfated (32%) galactofucan (Fuc:Gal = 2:1), with a Mw of 183 kDa (Mw/Mn = 2.0). Its supposed structure was found to be predominantly 1,3-linked fucose as the main chain, with branching points at C2 and C4. The branches could be single galactose and/or fucose short chains with terminal galactose residues. Sulfate groups were found at positions C3, C2, and/or C4 of fucose residues and at C2 and/or C4 of galactose residues. The radiosensitizing effect of galactofucans from S. oligocystum, S. duplicatum, and S. feldmannii against human melanoma SK-MEL-28, colon HT-29, and breast MDA-MB-231 cancer cells was investigated. The influence of all investigated polysaccharides treatments with/without X-ray radiation on colony formation of human melanoma cells SK-MEL-28 was weak. Fucoidan from S. feldmannii has been shown to be the most promising radiosensitizing compound against human colon HT-29 and breast MDA-MB-231 cancer cells. |
| Databáze: | OpenAIRE |
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